Immunization against PR8 influenza virus with chitosan-coated ISCOMATRIX nanoparticles

Mosafer, J. and Badiee, A. and Mohammadamini, Z. and Komeilinezhad, A. and Tafaghodi, M. (2018) Immunization against PR8 influenza virus with chitosan-coated ISCOMATRIX nanoparticles. Artificial Cells, Nanomedicine and Biotechnology, 46 (sup2). pp. 587-593.

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Abstract

Chitosan-coated ISCOMATRIX nanoparticles co-administrated with PR8 influenza virus were successfully developed via a lipid film hydration method to evaluate their in vivo immuniadjuvant potential in immunization against influenza. The prepared ISCOMATRIX (ISC) and chitosan-coated ISCOMATRIX (ISC-CIT) showed a particle size of 171 and 233 nm with a zeta potential of –9.47 and +5.65, respectively. Furthermore, ISC-CIT formulations were co-administered with PR8 antigen (PR8-ISC-CIT) and their immunogenicity was investigated after intranasal and intramuscular immunization of BALBc/mice. The PR8-ISC formulation elicited more IFN-γ after intranasal or intramuscular administration compared with PR8-ISC-CIT formulation. In contrast, although PR8-ISC-CIT formulation administered by intranasal route secreted more IFN-γ, it significantly decreased the IgG2a/IgG1 ratio and a less immune response was induced. Altogether, the ISC-adjuvanted influenza PR8 antigen could be considered as a powerful intramuscular antigen delivery system for producing a variety of prophylactic and therapeutic vaccines. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 Correspondence Address: Tafaghodi, M.; Nanotechnology Research Center, Mashhad University of Medical Sciences, P.O. Box 9196773117, Iran; email: Tafaghodim@mums.ac.ir
Uncontrolled Keywords: chitosan-coated ISCOMATRIX Immune response ISCOMATRIX nasal immunization PR8 influenza virus Antigens Chitosan Immune system Nanoparticles Particle size Viruses Antigen delivery system Hydration method Immunogenicity Influenza virus Intramuscular administration Therapeutic vaccines Immunization chitosan nanoparticle chlormethine gamma interferon immunoglobulin G1 immunoglobulin G2a influenza vaccine cholesterol cytokine immunological adjuvant nanoparticle phospholipid saponin animal cell animal experiment antibody response Article controlled study cytokine production drug delivery system drug formulation enzyme linked immunosorbent assay in vitro study in vivo study influenza influenza A Influenza A virus (A/Puerto Rico/8/1934(H1N1)) influenza vaccination intramuscular drug administration lipid membrane molecular weight mouse nonhuman photon correlation spectroscopy transmission electron microscopy zeta potential animal chemistry drug combination immunology male metabolism Adjuvants, Immunologic Animals Cytokines Drug Combinations Drug Compounding Influenza Vaccines Mice Phospholipids Saponins
Subjects: WC Communicable Diseases
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 08 May 2020 16:25
Last Modified: 08 May 2020 16:25
URI: http://eprints.mums.ac.ir/id/eprint/17427

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