Naegleria species population found in pond water of parks in Mashhad city, Can the physicochemical factors affect it?

Najafpoor, A. A. and Zarrinfar, H. and Ghaderifar, S. and Alidadi, H. and Esmaily, H. and Hajialilo, E. and Hosseini Farash, B. R. and Ahmadi, E. (2018) Naegleria species population found in pond water of parks in Mashhad city, Can the physicochemical factors affect it? MethodsX, 5. pp. 1427-1430.

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Abstract

Naegleria species are the ubiquitous free-living amoebas that are found worldwide in soil and water. Among Naegleria spp., N. fowleri can cause primary amebic meningoencephalitis (PAM). Ninety water samples were collected from the pond of parks. Also, the water quality parameters were measured at the sampling site (such as temperature, pH, total dissolved solids (TDS), electrical conductivity (EC) and Turbidity). After filtering, the samples were cultured on Bacto-agar enriched with Escherichia coli. A PCR assay was conducted on the culture-positive samples in the ITS1, 5.8SrDNA and ITS2 regions, and then the PCR products were sequenced. The pond water of parks was contaminated with some Naegleria spp. (except N. fowleri) and a Vahlkampfia avara. There was no significant relationship between water quality parameters and the presence of Naegleria (p > 0.05). Our protocol investigates to detect Naegleria spp. from ponds water of parks in Mashhad city and the relations between the water quality parameters and its presence. © 2018

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Ghaderifar, S.; Department of Environmental Health, Faculty of Health, Mashhad University of Medical SciencesIran; email: ghaderifarsh@gmail.com
Uncontrolled Keywords: Naegleria Naegleria species population found in pond water of parks in Mashhad city, Can the physicochemical factors affect it? Physicochemical factors Pond water lake water Article electric conductivity Iran nonhuman pH polymerase chain reaction priority journal temperature total dissolved solid turbidity Vahlkampfia Vahlkampfia avara water analysis
Subjects: WA Public Health
QX Parasitology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 07 May 2020 13:26
Last Modified: 07 May 2020 13:26
URI: http://eprints.mums.ac.ir/id/eprint/17441

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