Deregulated expression of HDAC3 in colorectal cancer and its clinical significance

Nemati, M. and Ajami, N. and Estiar, M. A. and Rezapour, S. and Gavgani, R. R. and Hashemzadeh, S. and Kafil, H. S. and Sakhinia, E. (2018) Deregulated expression of HDAC3 in colorectal cancer and its clinical significance. Advances in Clinical and Experimental Medicine, 27 (3). pp. 305-311.

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Abstract

Background. To date, 4 classes of histone deacetylases (HDACs) have been identified in humans. Class I HDACs are zinc-dependent and NAD + -independent enzymes, and include 4 isoforms closely related to yeast RPD3: HDAC1, 2, 3, and 8. Objectives. The aims of the study were to quantitatively evaluate the expression of HDAC3 in colorectal cancer (CRC) and to correlate its expression levels with clinicopathological parameters. Material and methods. We characterized expression patterns of HDAC3 as class I HDAC isoforms in a cohort of 48 CRC patients by quantitative (real-time) reverse transcription polymerase chain reaction (RT-PCR). In addition, the potential relationship between HDAC3 expression levels and clinicopathological parameters in patients suffering from CRC was explored. Results. We found that HDAC3 was highly expressed in colorectal tumors compared to normal colorectal tissues (p < 0.05). Furthermore, we found significant correlations between HDAC3 expression levels and tumor differentiation grades (p < 0.05). Conclusions. In this prospective study we identified a pronounced HDAC3 expression pattern in CRC. Our findings support an important role of HDAC3 as a complementary molecular marker for existing histopathological diagnostic elements; it might also have applications in prognostic and targeted therapy. Furthermore, HDAC3 can be used as a biomarker to differentiate between tumor borders and margins, and it may also be useful for characterizing field cancerization in CRC. © 2018 by Wroclaw Medical University.

Item Type: Article
Additional Information: Cited By :9 Export Date: 16 February 2020 Correspondence Address: Sakhinia, E.; Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical SciencesIran; email: esakhinia@yahoo.co.uk
Uncontrolled Keywords: Colorectal cancer Deregulation HDAC3 Prognosis complementary DNA glyceraldehyde 3 phosphate dehydrogenase histone deacetylase 3 histone deacetylase adult Article cancer diagnosis cancer grading clinical article cohort analysis female gene expression human human tissue liver metastasis lymph node metastasis male protein expression reverse transcription polymerase chain reaction sensitivity and specificity tumor invasion cell proliferation colon tumor colorectal tumor disease exacerbation down regulation enzymology genetics metabolism pathology prospective study Colonic Neoplasms Colorectal Neoplasms Disease Progression Down-Regulation Histone Deacetylases Humans Prospective Studies Reverse Transcriptase Polymerase Chain Reaction
Subjects: WI Digestive System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 07 May 2020 07:30
Last Modified: 07 May 2020 07:30
URI: http://eprints.mums.ac.ir/id/eprint/17453

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