Modified PAMAM vehicles for effective TRAIL gene delivery to colon adenocarcinoma: in vitro and in vivo evaluation

Pishavar, E. and Attaranzadeh, A. and Alibolandi, M. and Ramezani, M. and Hashemi, M. (2018) Modified PAMAM vehicles for effective TRAIL gene delivery to colon adenocarcinoma: in vitro and in vivo evaluation. Artificial Cells, Nanomedicine and Biotechnology, 46 (sup3). S503-S513.

[img] Text
Modified PAMAM vehicles for effective TRAIL gene delivery to colon adenocarcinoma in vitro and in vivo evaluation.pdf

Download (2MB)

Abstract

TRAIL (tumour necrosis factor-related apoptosis-inducing ligand) gene therapy is considered as one of the promising approaches for cancer treatment. Polyamidoamine (PAMAM) is one of the most extensively applied polymeric vector in gene delivery. In the current study, PAMAM (G4 and G5) dendrimers were modified with alkyl-carboxylate chain, PEG and cholesteryl chloroformate in order to enhance transfection efficiency through overcoming extracellular and intracellular barriers while reducing PAMAM cytotoxicity. Gene delivery efficiency of synthetized vectors was evaluated by both GFP (green fluorescent protein) reporter gene and TRAIL plasmid in colon cancer cells, in vitro and in vivo. The obtained results demonstrated that PAMAM G4-alkyl-PEG (3)-Chol (5)-TRAIL complexes at C/P ratio 4 could significantly increase cell death (29.45) in comparison with unmodified PAMAM vector (15.5). Moreover, in vivo study in C26 tumor-bearing BALB/c mice suggested that the prepared non-toxic safe vector could inhibit the tumor growth. This study represented the potent vehicle based on cholesterol-grafted PAMAM dendrimers with alkyl-PEG modification for efficient gene delivery in vitro and in vivo. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Cited By :3 Export Date: 16 February 2020 Correspondence Address: Ramezani, M.; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical SciencesIran; email: ramezanim@mums.ac.ir
Uncontrolled Keywords: cholesterol gene delivery Polyamidoamine polyethylene glycol TRAIL plasmid Carboxylation Cell death Dendrimers Diseases DNA Efficiency Gene transfer Mammals Polyethylene glycols Polyethylene oxides Tumors Vectors Cholesteryl chloroformate Colon cancer cells Green fluorescent protein Necrosis factors Polyamidoamines Transfection efficiency Gene therapy dendrimer drug vehicle tumor necrosis factor related apoptosis inducing ligand PAMAM Starburst Tnfsf10 protein, mouse animal cell animal experiment animal model animal tissue Article atomic force microscopy cell viability colon adenocarcinoma colon cancer cell line colorimetry comparative study controlled study cytotoxicity flow cytometry gene targeting genetic transfection heart in vitro study in vivo study kidney liver lung mouse nonhuman particle size plasmid proton nuclear magnetic resonance reporter gene spleen tumor growth tumor volume zeta potential adenocarcinoma animal Bagg albino mouse biosynthesis chemistry colon tumor evaluation study female genetics metabolism pathology procedures tumor cell line Animals Cell Line, Tumor Colonic Neoplasms Gene Transfer Techniques Genetic Therapy Mice Mice, Inbred BALB C TNF-Related Apoptosis-Inducing Ligand
Subjects: QV pharmacology
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 06 May 2020 14:37
Last Modified: 06 May 2020 14:37
URI: http://eprints.mums.ac.ir/id/eprint/17465

Actions (login required)

View Item View Item