Rheum turkestanicum reduces glutamate toxicity in PC12 and N2a cell lines

Rajabian, A. and Sadeghnia, H. R. and Moradzadeh, M. and Hosseini, A. (2018) Rheum turkestanicum reduces glutamate toxicity in PC12 and N2a cell lines. Folia Neuropathologica, 56 (4). pp. 354-361.

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Abstract

Glutamate is considered to be responsible for the pathogenesis of many neurodegenerative diseases. Reactive oxygen species (ROS) production is considered to be involved in the glutamate-induced apoptosis process. In this study, we investigated the neuroprotective effects of Rheum turkestanicum in the glutamate-induced rat pheochromocytoma (PC12 cells) and mouse neuroblastoma (N2a) cell lines. Rutin as an antioxidant was used as positive control. Glutamate cytotoxicity was accompanied by an increment of malondialdehyde (MDA) content, ROS generation and apoptosis induction. However, pretreatment with the root extract of R. turkestanicum significantly reduced MDA content, ROS generation and apoptotic cell death. Also rutin at a dose of 100 µM reduced ROS production and protected against glutamate toxicity. Also the quantification of rutin in R. turkestanicum extract was achieved and was about 0.11 ± 0.01 w/w. All these findings indicated that R. turkestanicum protected PC12 and N2a cells against glutamate-induced oxidative cell death and apoptosis and might raise the possibility of R. turkestanicum usage as a neuroprotective agent. © 2018 Termedia Publishing House Ltd. All Rights Reserved.

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 CODEN: FONEE Correspondence Address: Hosseini, A.; Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical SciencesIran; email: hosseiniaz1359@gmail.com
Uncontrolled Keywords: Apoptosis Glutamate N2a Oxidative damage PC12 Rheum turkestanicum Rutin glutamic acid malonaldehyde neuroprotective agent plant extract reactive oxygen metabolite Rheum turkestanicum extract rutoside unclassified drug antioxidant animal cell antioxidant activity Article controlled study high performance liquid chromatography lipid peroxidation mouse Neuro-2a cell line neuroapoptosis neuroprotection nonhuman PC12 cell line (pheochromocytoma) plant root rat Rheum animal cell death cell survival drug effect metabolism oxidative stress PC12 cell line Animals Antioxidants Neuroprotective Agents PC12 Cells Plant Extracts Rats Reactive Oxygen Species
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 06 May 2020 13:35
Last Modified: 06 May 2020 13:35
URI: http://eprints.mums.ac.ir/id/eprint/17480

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