Synergistic and defensive properties of Emblica officinalis, Terminalia chebula, and Terminalia bellerica extracts against serum/glucose deprivation-induced PC12 cells death

Rezvani, M. S. and Bakhtiari, E. and Tayarani-Najaran, Z. and Mousavi, S. H. (2018) Synergistic and defensive properties of Emblica officinalis, Terminalia chebula, and Terminalia bellerica extracts against serum/glucose deprivation-induced PC12 cells death. Jundishapur Journal of Natural Pharmaceutical Products, 13 (1).

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Synergistic and defensive properties of Emblica officinalis, Terminalia chebula, and Terminalia bellerica extracts against serumglucose deprivation-induced PC12 cells death.pdf

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Abstract

Background: Triphala as a combination of three plant's fruits including Emblica officinalis Gaertn, Terminalia chebula Retz, and Terminalia bellerica Roxb is valued for its antioxidant properties. It has been routinely used in various medicinal studies. Objective: In this study, the protective effects of E. officinalis, T. chebula, T. bellerica, and Triphala methanolic extracts on cell viability and reactive oxygen species (ROS) generated in PC12 cells were studied under serum/glucose deprivation (SGD) induced cell injury. Synergistic activity of three extracts was also explored. Methods: Cells were seeded overnight and then exposed to SGD condition for 18 h. Next, they were pretreated with different concentrations of the four extracts (3 -250 μg/ml) for 4 h. Cell viability and ROS generation were evaluated by viability assay and flow cytometry, respectively. Synergistic activity of three extracts was analyzed with Compusyn software. Results: SGD could induce cell toxicity after 18 h (P < 0.001). Pretreatment with extracts reduced SGD toxicity in PC12 cells. A significant raise in ROS generation was seen following SGD-induced toxicity. Pretreatment with T. chebula reversed the increased ROS production following ischemic insult. Conclusion: These results demonstrated the neuroprotection of these extracts possibly by ROS decrement and synergistic activity of them against SGD-induced toxicity. © 2018, Jundishapur Journal of Natural Pharmaceutical Products.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Mousavi, S.H.; Pharmacological Research Centre of Medicinal Plants, School of Medicine, Mashhad University of Medical SciencesIran; email: mousavih@mums.ac.ir
Uncontrolled Keywords: Cytotoxicity Ischemia PC12 Cells Emblica officinalis extract glucose natural product neuroprotective agent plant extract reactive oxygen metabolite Terminalia bellirica extract Terminalia chebula extract unclassified drug animal cell Article cell death cell proliferation cell viability concentration response controlled study drug potentiation Emblica officinalis flow cytometry glucose blood level MTT assay neuroprotection neurotoxicity nonhuman PC12 cell line rat Terminalia bellirica Terminalia chebula
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 06 May 2020 12:25
Last Modified: 06 May 2020 12:25
URI: http://eprints.mums.ac.ir/id/eprint/17491

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