The importance of stool DNA methylation in colorectal cancer diagnosis: A meta-analysis

Shariatpanahi, A. M. and Yassi, M. and Nouraie, M. and Sahebkar, A. and Tabrizi, F. V. and Kerachian, M. A. (2018) The importance of stool DNA methylation in colorectal cancer diagnosis: A meta-analysis. PLoS ONE, 13 (7).

[img] Text
The importance of stool DNA methylation in colorectal cancer diagnosis A meta-analysis.pdf

Download (6MB)


A large number of tumor-related methylated genes have been suggested to be of diagnostic and prognostic values for CRC when analyzed in patients’ stool samples; however, reported sensitivities and specificities have been inconsistent and widely varied. This meta-analysis was conducted to assess the detection accuracy of stool DNA methylation assay in CRC, early stages of CRC (advanced adenoma, non-advanced adenomas) and hyperplastic polyps, separately. We searched MEDLINE, Web of Science, Scopus and Google Scholar databases until May 1, 2016. From 469 publications obtained in the initial literature search, 38 studies were included in the final analysis involving 4867 individuals. The true positive, false positive, true negative and false negative of a stool-based DNA methylation biomarker using all single-gene tests considering a certain gene; regardless of a specific gene were pooled and studied in different categories. The sensitivity of different genes in detecting different stages of CRC ranged from 0 to 100 and the specificities ranged from 73 to 100. Our results elucidated that SFRP1 and SFRP2 methylation possessed promising accuracy for detection of not only CRC (DOR: 31.67; 95CI, 12.31–81.49 and DOR: 35.36; 95CI, 18.71–66.84, respectively) but also the early stages of cancer, adenoma (DOR: 19.72; 95 CI, 6.68–58.25 and DOR: 13.20; 95CI, 6.01–28.00, respectively). Besides, NDRG4 could be also considered as a significant diagnostic marker gene in CRC (DOR: 24.37; 95CI, 10.11–58.73) and VIM in adenoma (DOR: 15.21; 95CI, 2.72–85.10). In conclusion, stool DNA hypermethylation assay based on the candidate genes SFRP1, SFRP2, NDRG4 and VIM could offer potential diagnostic value for CRC based on the findings of this meta-analysis. © 2018 Mojtabanezhad Shariatpanahi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Item Type: Article
Additional Information: Cited By :8 Export Date: 16 February 2020 CODEN: POLNC Correspondence Address: Kerachian, M.A.; Cancer Genetics Research Unit, Reza Radiotherapy and Oncology CenterIran; email:
Uncontrolled Keywords: secreted frizzled related protein 1 secreted frizzled related protein 2 tumor marker membrane protein muscle protein NDRG4 protein, human nerve protein SFRP1 protein, human SFRP2 protein, human signal peptide vimentin Article cancer diagnosis cancer prognosis cancer staging colorectal cancer diagnostic accuracy diagnostic value DNA methylation assay false negative result false positive result feces analysis human meta analysis NDRG4 gene oncogene predictive value sensitivity and specificity SFRP1 gene SFRP2 gene VIM gene chemistry colorectal tumor DNA methylation early cancer diagnosis feces genetics Biomarkers, Tumor Colorectal Neoplasms Early Detection of Cancer Humans Intercellular Signaling Peptides and Proteins Membrane Proteins Muscle Proteins Nerve Tissue Proteins
Subjects: WI Digestive System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 04 May 2020 07:48
Last Modified: 04 May 2020 07:48

Actions (login required)

View Item View Item