Study of the role of efflux pumps in amikacin-resistant acinetobacter isolates from teaching hospitals of mashhad, Iran

Shaye, M. A. and Sharifmoghadam, M. R. and Bahreini, M. and Ghazvini, K. and Mafinezhad, A. and Amiri, G. (2018) Study of the role of efflux pumps in amikacin-resistant acinetobacter isolates from teaching hospitals of mashhad, Iran. Jundishapur Journal of Microbiology, 11 (4).

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Background: The development of multidrug-resistant Acinetobacter species has created serious problems in nosocomial infections. Understanding the underlying resistance mechanisms and their significance in conferring resistance to different antibiotics is the first step to develop strategies for fighting or reversing the current resistance. Objectives: The aim of this study was to investigate the role of efflux pumps in decreasing susceptibility to amikacin in Acinetobacter clinical isolates. Methods: Forty-six clinical Acinetobacter isolates were collected from 2 teaching hospitals of Mashhad, Iran. Susceptibility testing was conducted by the disc diffusion method. Amikacin minimum inhibitory concentration (MIC) for resistant Acinetobacter isolates was determined according to the Clinical and Laboratory Standards Institute (CLSI) guidelines either with or without the efflux pumps inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP). Conventional polymerase chain reaction (PCR) was used to analyze the presence of pump genes. Results: Acinetobacter isolates were identified as 2 species; Acinetobacter baumannii and A. lwoffii. Susceptibility testing showed high levels of resistance to amikacin in 27 isolates, including both A. baumannii and A. lwoffii, among which 20 A. baumannii isolates showed a 2- to 524288-fold reduction in amikacin MIC in the presence of CCCP, while no reduction occurred in amikacin MIC in resistant A. lwoffii isolates. The PCR results showed high frequencies of adeB, abeM, and adeI genes in Acinetobacter isolates yet the adeE gene was not found in any of the isolates. Conclusions: The obtained results indicated the importance of efflux pumps in conferring resistance to amikacin in clinical isolates of A.baumannii, yet not in A. lwoffii. © 2018, Jundishapur Journal of Microbiology.

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 Correspondence Address: Sharifmoghadam, M.R.; Department of Biology, Faculty of Science, Ferdowsi University of MashhadIran; email:
Uncontrolled Keywords: Acinetobacter baumannii Acinetobacter lwoffii Amikacin Antibiotic Resistance CCCP Efflux Pumps amoxicillin amoxicillin plus clavulanic acid ampicillin bacterial protein carbenicillin carbonyl cyanide chlorophenylhydrazone cefotaxime ceftazidime ceftriaxone ciprofloxacin efflux pump gentamicin imipenem piperacillin polymyxin B tetracycline derivative tobramycin unclassified drug abeM gene Acinetobacter Acinetobacter infection adeB gene adeE gene adeI gene antibiotic sensitivity Article bacterial gene disk diffusion human Iran minimum inhibitory concentration nonhuman polymerase chain reaction practice guideline protein function teaching hospital
Subjects: QU Biochemistry
QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 04 May 2020 08:11
Last Modified: 04 May 2020 08:11

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