Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System

Shoorei, H. and Khaki, A. and Ainehchi, N. and Hassanzadeh Taheri, M. and Tahmasebi, M. and Seyedghiasi, G. and Ghoreishi, Z. and Shokoohi, M. and Khaki, A. and Abbas Raza, S. (2018) Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System. Chinese Medical Journal, 131 (2). pp. 218-225.

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Abstract

Background: The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomilla (MC) on preantral follicle culture of mouse ovaries in a three-dimensional culture system. Methods: Isolated preantral follicles were randomly divided into three main groups: the control group containing 10 fetal bovine serum without MC extract (G1), the first experimental group supplemented with 25 μg/ml hydroalcoholic extract of chamomile (G2), and the second experimental group supplemented with 50 μg/ml hydroalcoholic extract of chamomile (G3). Results: After 12 days of culture, the survival rate (P < 0.05), antrum formation (P < 0.01), metaphase two oocytes (P < 0.01), and the expression of PCNA (P < 0.05) and FSHR (P < 0.05) genes significantly decreased in G3 as compared with G1. On the other hand, at the last day of culture (day 12), the mean diameter of follicles cultured in the medium which was supplemented with 50 μg/ml hydroalcoholic extract of chamomile significantly decreased as compared with the G1 (P < 0.05). In addition, the levels of progesterone and dehydroepiandrosterone hormones significantly increased in the medium of G3 relative to G1 (P < 0.01), while in the medium of G1, the level of 17β-estradiol was significantly higher than that of other groups (P < 0.01). Reactive oxygen species levels of metaphase II oocytes were significantly decreased in G2 as compared with G1 (P < 0.01). Conclusion: Adding chamomile extract to culture media appeared to decrease follicular function and development. © 2018 Chinese Medical Journal Produced by Wolters Kluwer-Medknow.

Item Type: Article
Additional Information: Cited By :11 Export Date: 16 February 2020 CODEN: CMDJA Correspondence Address: Khaki, A.; Department of Pathology, Tabriz Branch, Islamic Azad UniversityIran; email: arashkhaki@yahoo.com
Uncontrolled Keywords: Follicular Development Follicular Maturation FSHR Gene PCNA Gene Steroid Hormones Toxicology cycline estradiol follitropin receptor Matricaria chamomilla extract prasterone progesterone reactive oxygen metabolite plant extract animal tissue Article controlled study drug effect female gene expression in vitro study metaphase mouse nonhuman oocyte ovary follicle development ovary follicle maturation ovulation induction animal cell culture genetics growth, development and aging Matricaria metabolism ovary follicle real time polymerase chain reaction Animals Cells, Cultured Mice Ovarian Follicle Plant Extracts Proliferating Cell Nuclear Antigen Reactive Oxygen Species Real-Time Polymerase Chain Reaction Receptors, FSH
Subjects: WP Gynecology
QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 04 May 2020 03:44
Last Modified: 04 May 2020 03:44
URI: http://eprints.mums.ac.ir/id/eprint/17538

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