HTLV-1-host interactions on the development of adult T cell leukemia/lymphoma: Virus and host gene expressions

Tarokhian, H. and Rahimi, H. and Mosavat, A. and Shirdel, A. and Rafatpanah, H. and Akbarin, M. M. and Bari, A. and Ramezani, S. and Rezaee, S. A. (2018) HTLV-1-host interactions on the development of adult T cell leukemia/lymphoma: Virus and host gene expressions. BMC Cancer, 18 (1).

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Abstract

Background: Adult T-cell leukemia/lymphoma (ATLL) is a lymphoproliferative disorder of HTLV-1-host interactions in infected TCD4+ cells. In this study, the HTLV-1 proviral load (PVL) and HBZ as viral elements and AKT1, BAD, FOXP3, RORγt and IFNλ3 as the host factors were investigated. Methods: The study was conducted in ATLLs, HTLV-1-associated myelopathy/tropical spastic paraparesis patients (HAM/TSPs) and HTLV-1-asympthomatic carriers (ACs). The DNA and mRNA from peripheral blood mononuclear cells were extracted for gene expression assessments via qRT-PCR, TaqMan assay, and then confirmed by western blotting. Results: As it was expected, the HTLV-1-PVL were higher in ATLLs than ACs (P = 0.002) and HAM/TSP (P = 0.041). The HBZ expression in ATLL (101.76 ± 61.3) was radically higher than in ACs (0.12 ± 0.05) and HAM/TSP (0.01 ± 0.1) (P = 0.001). Furthermore, the AKT1 expression in ATLLs (13.52 ± 4.78) was higher than ACs (1.17 ± 0.27) (P = 0.05) and HAM/TSPs (0.72 ± 0.49) (P = 0.008). However, BAD expression in ATLL was slightly higher than ACs and HAM/TSPs and not significant. The FOXP3 in ATLLs (41.02 ± 24.2) was more than ACs (1.44 ± 1) (P = 0.007) and HAM/TSP (0.45 ± 0.15) (P = 0.01). However, RORγt in ATLLs (27.43 ± 14.8) was higher than ACs (1.05 ± 0.32) (P = 0.02) but not HAM/TSPs. Finally, the IFNλ3 expression between ATLLs (31.92 ± 26.02) and ACs (1.46 ± 0.63) (P = 0.01) and ACs and HAM/TSPs (680.62 ± 674.6) (P = 0.02) were statistically different, but not between ATLLs and HAM/TSPs. Conclusions: The present and our previous study demonstrated that HTLV-1-PVL and HBZ and host AKT1 and Rad 51 are novel candidates for molecular targeting therapy of ATLL. However, high level of RORγt may inhibit Th1 response and complicated in ATLL progressions. © 2018 The Author(s).

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020 CODEN: BCMAC Correspondence Address: Rezaee, S.A.; Immunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Azadi-Square, Medical CampusIran; email: RezaeeR@mums.ac.ir
Uncontrolled Keywords: AKT1 BAD FOXP3 HBZ HTLV-1-proviral load IFNλ3 RORγt interferon interferon lamda 3 messenger RNA protein BAD protein kinase B Rad51 protein retinoid related orphan receptor gamma transcription factor FOXP3 unclassified drug virus DNA AKT1 protein, human BAD protein, human basic leucine zipper transcription factor forkhead transcription factor FOXP3 protein, human HBZ protein, human T-cell leukemia virus type I RORC protein, human viral protein adult AKT1 gene Article BAD gene clinical article controlled study cross-sectional study disease association female FOXP3 gene gene expression HBZ gene human human cell Human T-lymphotropic virus 1 innate immunity male middle aged nonhuman peripheral blood mononuclear cell protein expression provirus quantitative analysis real time polymerase chain reaction ROR gamma t gene T cell leukemia tropical spastic paraparesis virus carrier virus cell interaction virus gene virus load Western blotting blood gene expression regulation genetics host pathogen interaction HTLV-1 infection metabolism mononuclear cell pathogenicity pathology Th1 cell virology Basic-Leucine Zipper Transcription Factors bcl-Associated Death Protein Forkhead Transcription Factors Gene Expression Regulation, Neoplastic Gene Expression Regulation, Viral Host-Pathogen Interactions HTLV-I Infections Humans Leukemia-Lymphoma, Adult T-Cell Leukocytes, Mononuclear Nuclear Receptor Subfamily 1, Group F, Member 3 Paraparesis, Tropical Spastic Proto-Oncogene Proteins c-akt Rad51 Recombinase Retroviridae Proteins RNA, Messenger Th1 Cells Viral Load
Subjects: WH Hemic and Lymphatic System
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 27 Apr 2020 08:29
Last Modified: 27 Apr 2020 08:29
URI: http://eprints.mums.ac.ir/id/eprint/17557

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