Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015-2016

Tavanaee Sani, A. and Ashna, H. and Kaffash, A. and Khaledi, A. and Ghazvini, K. (2018) Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015-2016. Ethiopian journal of health sciences, 28 (3). pp. 299-304.

[img] Text
Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015-2016.pdf

Download (206kB)

Abstract

Background: Drug resistance is a leading concern in control of TB. Resistance against rifampin as one of the most important drugs in the treatment of Mycobacterium tuberculosis is caused by mutations in the 81-base pair region of the rpoB gene encoding the β-subunit of RNA polymerase. This study aimed to characterize the mutations in the rpoB gene associated with rifampin resistance among M. tuberculosis. Methods: This study was conducted on referred samples of patients who did not respond to anti-TB treatment, in Tuberculosis Regional Reference Laboratory at Shariati Hospital. Drug susceptibility of M. tuberculosis isolates was surveyed using a proportional method on LJ medium. The isolates with resistant to rifampin were reconfirmed and then the rpoB gene was amplified and sequenced. Results: Among 27 resistant cases, 8, 11 and 8 people were from Iran, Afghanistan, and Turkmenistan, respectively. In 26 out of 27 isolates, rpoB gene mutations were observed. The most prevalent mutations belonged to the codon 53. The most prevalent mutations belonged to the TCG (Ser) 531TTG (leu) with prevalence 51.8 (n=14), and GAC (Asp)516TAC (Tyr), CAC (His) 526GAC (Asp) and CAC (His) 526TAC(Tyr) mutations with prevalence 14.8(n=4). Twenty-three isolates had just one mutation. Conclusion: The use of rpoB gene sequencing led to the lack of the need for growth of the organism in the culture medium, the direct use of clinical samples, reduction of biological risks and a detection about 96.3 of MDR TB cases lowering the cost of the treatment.

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020
Uncontrolled Keywords: Drug-resistant Mycobacterium tuberculosis Rifampicin rpoB gene bacterial protein DNA directed RNA polymerase rpoB protein, Mycobacterium tuberculosis tuberculostatic agent adult antibiotic resistance bacterial gene codon drug effect female genetics growth, development and aging human Iran male microbial sensitivity test microbiology middle aged multidrug resistant tuberculosis mutation tuberculosis young adult Antitubercular Agents Bacterial Proteins DNA-Directed RNA Polymerases Drug Resistance, Bacterial Genes, Bacterial Humans Microbial Sensitivity Tests Rifampin Tuberculosis, Multidrug-Resistant
Subjects: WC Communicable Diseases
WF Respiratory System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 27 Apr 2020 08:51
Last Modified: 27 Apr 2020 08:51
URI: http://eprints.mums.ac.ir/id/eprint/17559

Actions (login required)

View Item View Item