Genotypic diversity of multidrug resistant Shigella species from Iran

Zamanlou, S. and Rezaee, M. A. and Aghazadeh, M. and Ghotaslou, R. and Nave, H. H. and Khalili, Y. (2018) Genotypic diversity of multidrug resistant Shigella species from Iran. Infection and Chemotherapy, 50 (1). pp. 29-37.

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Abstract

Background: In many developing countries, shigellosis is endemic and also occurs in epidemics and treatment of multidrug-resistant (MDR) isolates are important. The aims of this study were to determine the antimicrobial susceptibility, prevalence of class 1 and 2 integrons and the clonal relatedness of isolates. Materials and Methods: Antimicrobial susceptibility tests were performed by disc diffusion method. Polymerase chain reaction (PCR)-sequencing technique was employed for detection and characterization of integrons. The genetic relatedness was evaluated by using enterobacterial repetitive intergenic consensus (ERIC) PCR. Results: There was a high percentage of resistance to trimethoprim-sulfamethoxazole (TMP/SMX) (93.7), ampicillin (AMP) (87.3), streptomycin (STR) (84.5) and tetracycline (TET) (78.9). MDR phenotype was seen in 95.1 of total isolates. Most common MDR profile was TMP/SMX/STR/AMP resistant pattern. Among the 142 Shigella spp. analyzed in this study, 28 isolates were positive for class 1 integron with two types of gene cassette arrays (dfrA17/aadA5 = 31.7 and dfrA7 = 3.8). The class 2 integron was more frequently detected among the isolates (94.7) with dfrA1/sat1/aadA1 (69.4) and dfrA1/sat1 (30.6) gene cassettes. ERIC-PCR results showed 6, 5, 4 and 3 main genotypes among S. flexneri, S. sonnei, S. boydii and S. dysenteriae isolates, respectively. Conclusion: Our findings revealed that multidrug resistant Shigella species with high prevalence of class 2 integron were very common in Iran. In addition, ERIC-PCR patterns showed limited variety of clones are responsible for shigellosis in the region of the study. © 2018 by The Korean Society of Infectious Diseases, Korean Society for Chemotherapy.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Rezaee, M.A.; Immunology Research Center, Tabriz University of Medical SciencesIran; email: rezaee@tbzmed.ac.ir
Uncontrolled Keywords: Enterobacterial repetitive intergenic consensus Integron Multidrug resistant Polymerase chain reaction Shigella amikacin amoxicillin plus clavulanic acid ampicillin azithromycin aztreonam bacterial DNA cefazolin cefepime cefotaxime cefoxitin ceftazidime ceftriaxone chloramphenicol ciprofloxacin cotrimoxazole genomic DNA gentamicin imipenem levofloxacin meropenem nalidixic acid ofloxacin streptomycin tetracycline aadA1 gene aadA5 gene adolescent adult antibiotic resistance Article bacterial gene bacterium isolate child controlled study dfrA1 gene dfrA17 gene dfrA7 gene female gene cassette genetic variability genotype human Iran male multidrug resistance nonhuman phenotype sat1 gene sequence analysis Shigella boydii Shigella dysenteriae Shigella flexneri Shigella sonnei shigellosis
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: lib2 lib2 lib2
Date Deposited: 27 Apr 2020 05:12
Last Modified: 27 Apr 2020 05:12
URI: http://eprints.mums.ac.ir/id/eprint/17586

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