Occult hepatitis B virus among the patients with abnormal alanine transaminase

Makvandi, M. and Neisi, N. and Khalafkhany, D. and Makvandi, K. and Hajiani, E. and Shayesteh, A. A. and Zadeh, A. M. and Sina, A. H. and Hamidifard, M. and Rasti, M. and Aryan, E. and Ahmadi, K. and Yad, M. J. Y. (2014) Occult hepatitis B virus among the patients with abnormal alanine transaminase. Jundishapur Journal of Microbiology, 7 (8).

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Abstract

Background: The occult hepatitis B infection (OBI) is defined as the presence of hepatitis B virus (HBV) DNA in the sera or in the liver biopsy and the absence of hepatitis B surface antigen (HBsAg) by serological test. Objectives: The current study aimed to evaluate the occult HBV infection by polymerase chain reaction (PCR) and determine HBV genotyping among the patients with abnormal alanine transaminase (ALT) in Ahvaz city, Iran. Patients and Methods: The sera of 120 patients, 54 (45) females and 66 (55) males, with abnormal ALT 40-152 IU were collected. All the patients were negative for HBsAg for more than one year. The patients' sera were tested by PCR using primers specified for the S region of HBV. Then the positive PCR products were sequenced to determine HBV genotyping and phylogenic tree. Results: Of these 120 subjects, 12 (10) patients including 6 (5) males and 6 (5) females were found positive for HBV DNA by PCR, which indicated the presence of occult HBV infection among these patients. The sequencing results revealed that genotype D was predominant with sub-genotyping D1 among OBI patients. Conclusions: Occult hepatitis B infection is remarkably prevalent in Ahvaz, Iran, and should be considered as a potential risk factor for the transmission of Hepatitis B Virus throughout the community by the carriers. © 2014, Ahvaz Jundishapur University of Medical Sciences; Published by Kowsar Corp.

Item Type: Article
Additional Information: Cited By :3 Export Date: 16 February 2020 Correspondence Address: Makvandi, M.; Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; email: manoochehrmakvandi29@yahoo.com
Uncontrolled Keywords: Alanine transaminase Hepatitis b Occult Polymerase chain reaction alanine aminotransferase hepatitis B surface antigen immunoglobulin G virus DNA adult article controlled study female groups by age Hepatitis B virus genotype D human Iran major clinical study male middle aged nucleotide sequence phylogenetic tree prevalence sequence analysis virus detection virus identification young adult
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib6 lib6
Date Deposited: 04 Nov 2020 08:13
Last Modified: 04 Nov 2020 08:13
URI: http://eprints.mums.ac.ir/id/eprint/17830

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