PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells

Darvishi, Marzieh and Ghasemi Hamidabadi, Hatef and Sahab Negah, Sajad and Moayeri, Ardeshir and Tiraihi, Taki and Mirnajafi-Zadeh, Javad and Jahanbazi Jahan-Abad, Ali and Shojaei, Amir (2020) PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells. Iranian Journal of Basic Medical Sciences, 23 (4). pp. 431-438.

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Official URL: http://ijbms.mums.ac.ir/article_14926.html

Abstract

Objective(s): Cell therapy has provided clinical applications to the treatment of motor neuron diseases. The current obstacle in stem cell therapy is to direct differentiation of stem cells into neurons in the neurodegenerative disorders. Biomaterial scaffolds can improve cell differentiation and are widely used in translational medicine and tissue engineering. The aim of this study was to compare the efficiency of two-dimensional with a three-dimensional culture system in their ability to generate functional motor neuron-like cells from adipose-derived stem cells. Materials and Methods: We compared motor neuron-like cells derived from rat adipose tissue in differentiation, adhesion, proliferation, and functional properties on two-dimensional with three-dimensional culture systems. Neural differentiation was analyzed by immunocytochemistry for immature (Islet1) and mature (HB9, ChAT, and synaptophysin) motor neuron markers. Results: Our results indicated that the three-dimensional environment exhibited an increase in the number of Islet1. In contrast, two-dimensional culture system resulted in more homeobox gene (HB9), Choline Acetyltransferase (ChAT), and synaptophysin positive cells. The results of this investigation showed that proliferation and adhesion of motor neuron-like cells significantly increased in three-dimensional compared with two-dimensional environments. Conclusion: The findings of this study suggested that three-dimension may create a proliferative niche for motor neuron-like cells. Overall, this study strengthens the idea that three-dimensional culture may mimic neural stem cell environment for neural tissue regeneration.

Item Type: Article
Uncontrolled Keywords: Motor neuron-like cells,Nanoscaffolds,Proliferation,Stem cell therapy,Three-dimension culture,Tissue engineering
Subjects: QV pharmacology
Divisions: Journals > Iranian J Basic Medical Sciences
Depositing User: ijbms ijbms
Date Deposited: 24 Jun 2020 05:15
Last Modified: 24 Jun 2020 05:15
URI: http://eprints.mums.ac.ir/id/eprint/18035

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