Pomegranate peel extract inhibits internalization and replication of the influenza virus: An in vitro study

Moradi, Mohammad-Taghi and Karimi, Ali and Rafieian-kopaei, Mahmoud and Rabiei-Faradonbeh, Mohammad and Momtaz, Hassan (2020) Pomegranate peel extract inhibits internalization and replication of the influenza virus: An in vitro study. Avicenna Journal of Phytomedicine, 10 (2). pp. 143-151.

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Abstract

Objective: Influenza virus, which is associated with high level of morbidity and mortality, has been recently considered a public health concern; however, the methods of choice to control and treat it are limited. Our previous study showed anti-influenza virus activity of pomegranate peel extract (PPE). In this study, the mechanism through which PPE acts against influenza virus A/Puerto Rico/8/34 (H1N1; PR8) was investigated. Materials and Methods:Ethyl alcohol extract of pomegranate (Punica granatum L.) peel was prepared, and the action mechanism of PPE in inhibiting influenza replication was studied by time-of-drug-addition assay, virucidal activity, RNA replication, hemagglutination inhibition assay, viral mRNA expression, and western blot analysis. Results: PPE inhibited viral polymerase activity, viral RNA replication, and viral protein expression but could not affect hemagglutination inhibition and virucidal activity. According to time-of-drug-addition assay results, PPE inhibited the virus adsorption and early steps of influenza replication. Conclusion: This study demonstrated that the antiviral effect of PPE on influenza virus is most probably associated with inhibition of viral adsorption and viral RNA transcription.

Item Type: Article
Uncontrolled Keywords: Anti-influenza virus,Pomegranate,Punica granatum L,Mechanisms
Subjects: QT physiology
QU Biochemistry
QV pharmacology
QW Microbiology and Immunology
Divisions: Journals > Avicenna J Phytomedicine
Depositing User: ajp ajp
Date Deposited: 08 Apr 2020 09:51
Last Modified: 08 Apr 2020 09:51
URI: http://eprints.mums.ac.ir/id/eprint/18211

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