Photosensitizer effects of MWCNTs-COOH particles on CT26 fibroblastic cells exposed to laser irradiation

Abdi Goushbolagh, N. and Keshavarz, M. and Zare, M. H. and Bahreyni-Toosi, M. H. and Kargar, M. and Farhood, B. (2019) Photosensitizer effects of MWCNTs-COOH particles on CT26 fibroblastic cells exposed to laser irradiation. Artificial Cells, Nanomedicine and Biotechnology, 47 (1). pp. 1326-1334.

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Abstract

In the current study, photosensitizer effect of carboxylate multiwalled carbon nanotubes (MWCNTs-COOH) on CT26 fibroblastic cells following near infrared (NIR) irradiation was quantized in photo-thermal therapy (PTT). Moreover, it was tried to achieve optimal dose of MWCNTs-COOH and laser exposure time. Characterizations of MWCNTs-COOH were scrutinized using scanner electron microscope (SEM), spectrophotometer, and particle size analyzer. The seeded CT26 cells were treated with nontoxic concentrations of MWCNTs-COOH and then irradiated. Finally, viability () of the CT26 cells was determined using MTT assay. The findings revealed that 10, 50, and 80 μg/mL of MWCNTs-COOH have remarkable photosensitizer effects on CT26 cancerous cell lines against NIR irradiation (2.5 W/cm2). It was shown that using the 80 μg/mL concentration of MWCNTs-COOH against 60, 120, 180, 240, and 300 s of NIR irradiation and also, 10 and 50 μg/mL concentration of MWCNTs-COOH against 180, 240, and 300 s of NIR irradiation can lead to significant decrease in mean cell viability () by more than 50. According to the obtained data, it seems that using the PPT with MWCNTs-COOH, as adjunct therapy in CT26 fibroblastic cells, can help to increase therapeutic ratio of main modalities of cancer treatment such as radiotherapy, chemotherapy, and surgery. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Farhood, B.; Department of Medical Physics, Faculty of Medical sciences, Shahid Sadoughi University of SciencesIran; email: mhzare2009@gmail.com
Uncontrolled Keywords: carboxylate muli-wall carbon nanotubes (MWCNTs-COOH) CT26 cells NIR irradiation photosensitizer Photothermal-therapy Carboxylation Cell culture Cells Chemotherapy Infrared devices Irradiation Nanotubes Particle size Particle size analysis Photosensitizers Radiation Yarn Cancerous cells Fibroblastic cells Nontoxic concentrations Particle size analyzers Photothermal therapy Scanner electron microscopes Therapeutic ratios Multiwalled carbon nanotubes (MWCN) carboxylic acid distilled water multi walled nanotube photosensitizing agent carbon nanotube absorption spectroscopy animal cell Article cell proliferation cell viability controlled study CT26 cell line cytotoxicity mouse MTT assay nonhuman photosensitization radiation exposure scanning electron microscopy temperature ultraviolet visible spectroscopy zeta potential cell line cell survival chemistry cytology drug effect fibroblast human low level laser therapy radiation response Carboxylic Acids Fibroblasts Humans Low-Level Light Therapy Nanotubes, Carbon Photosensitizing Agents
Subjects: QT physiology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 04:58
Last Modified: 21 Jun 2020 04:58
URI: http://eprints.mums.ac.ir/id/eprint/18220

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