Autophagy induction plays time-dependent role in viral load of HCV infected Huh7.5 cell line

Abdoli, M. and Abdoli, A. and Aryan, E. and Meshkat, Z. (2019) Autophagy induction plays time-dependent role in viral load of HCV infected Huh7.5 cell line. IUBMB Life, 71 (1). pp. 41-44.

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Abstract

Autophagy provides an initial membranous platform for incoming hepatitis C virus (HCV) RNA translation and immune evasion. Once HCV replication is established, this infrastructure will be unnecessary for translation of HCV RNA progeny. So, the autophagy plays key role in the replication and immune pathogenesis of HCV virus. The aim of this study was to study the effect of autophagy induction in Huh7.5 cell on virus titer. The Huh7.5 cell was transfected with recombinant pcDNA-Beclin1. The autophagy induction was evaluated via microtubule associated protein 1 light chain 3 staining as autophagy formation marker using flow cytometry. The HCV (JFH1) was inoculated 12-h post-transfection. Next, to evaluate the viral load, viral RNA was extracted after 24 and 48 h and virus titer was calculated using real-time PCR. The result of the current study shows that the induction of autophagy before virus infection was able to enhance virus yield from 4 × 103 copies/mL to 1 × 104 copies/mL at 24-h post-infection, but reduced viral load after 48 h up to 6 × 103 copies/mL. The study of cross-talk between autophagy and HCV may bring new hope for human intervention and treatment of HCV. Also, it opens new avenue to improve virus cultivation in cell culture and understanding HCV and host cell responses. © 2018 IUBMB Life, 71(1):41–44, 2019. © 2018 International Union of Biochemistry and Molecular Biology

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 CODEN: IULIF Correspondence Address: Meshkat, Z.; Antimicrobial Resistance Research Center, Mashhad University of Medical ScienceIran; email: meshkatz@mums.ac.ir
Uncontrolled Keywords: autophagy HCV Huh7.5 cell beclin 1 microtubule associated protein 1 microtubule associated protein 1 light chain 3 unclassified drug virus RNA Article controlled study flow cytometry genetic transfection hepatitis C Hepatitis C virus nonhuman real time polymerase chain reaction virus culture virus load Western blotting genetics Hepacivirus human liver cell pathogenicity pathology tumor cell line virology virus replication Beclin-1 Cell Line, Tumor Hepatocytes Humans RNA, Viral Transfection Viral Load
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 04:58
Last Modified: 21 Jun 2020 04:58
URI: http://eprints.mums.ac.ir/id/eprint/18221

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