Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG: as a potential delivery system for vaccine development

Amel Jamehdar, S. and Tabaei, S. and Mashkani, B. and Karimi, R. and Motallebnezhad, M. and Esmaili, A. (2019) Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG: as a potential delivery system for vaccine development. Bioengineered, 10 (1). pp. 689-696.

[img] Text
Construction of Epstein-Bar virus cocktail peptide fused with Fcγ of IgG as a potential delivery system for vaccine development.pdf

Download (1MB)

Abstract

Epstein–Barr virus (EBV) associated with several diseases such as contagious mononucleosis chronic active EBV infection, and diverse sorts of malignant tumors. Therefore, using applicable vaccines could be advantageous for public health. Yet, the vaccine has been unavailable to protect from EBV so far. In the current study, to develop a multi-peptide vaccine for EBV and assess its expression in Pichia pastoris yeast system, three immunodominant sequences in glycoprotein (gp) 85, gp350 and latent membrane protein 1 (LMP1) were chosen. To construct fusion peptide, -GGGGS- liker was applied. After cloning the fusion peptide in the pPICZαA expression vector, this recombinant vector processed and transfected into Pichia pastoris host cells. The expression of high level of EBV fusion peptide was confirmed by dot blot and SDS-PAGE procedures. The Pichia pastoris is capable of supporting EBV fusion peptide expression. The application of this fusion peptide as a peptide vaccine to fight EBV is suggested. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Esmaili, A.; Department of Biology, Damghan Branch, Islamic Azad UniversityIran; email: arezooesmaili11@yahoo.com
Uncontrolled Keywords: Epstein-Bar virus fusion peptide peptide vaccine Pichia pastoris
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 20 Jun 2020 09:20
Last Modified: 20 Jun 2020 09:20
URI: http://eprints.mums.ac.ir/id/eprint/18248

Actions (login required)

View Item View Item