Upregulation of glutamate transporter 1 by clavulanic acid administration and attenuation of allodynia and hyperalgesia in neuropathic rats

Amin, B. and Avaznia, M. and Noorani, R. and Mehri, S. and Hosseinzadeh, H. (2019) Upregulation of glutamate transporter 1 by clavulanic acid administration and attenuation of allodynia and hyperalgesia in neuropathic rats. Basic and Clinical Neuroscience, 10 (4). pp. 345-354.

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Abstract

Introduction: Clavulanic acid (CLAV) is structurally similar to ceftriaxone, a potent stimulator of glial GlutamateTransporter-1 (GLT-1) expression. The present study aims at exploring the anti-nociceptive effects of CLAV, a beta-lactamase inhibitor in rats underwent sciatic nerve Chronic Constriction Injury (CCI). Methods: CLAV (12.5, 25, 50 mg/kg) was administered intraperitoneally after the surgery for 14 consecutive days. Behavioral pain parameters were evaluated before and 3, 5, 7, 10 and 14 days after injury. Spinal GLT-1 level was measured via western blotting at days 7 and 14. Results: CCI led to mechanical allodynia, cold allodynia and thermal hyperalgesia which started on postoperative days 3 and continued until the end of study. We found that CLAV (12.5 and 25 mg/kg) significantly attenuated all pain related behaviors as compared to the CCI animals treated with normal saline. Protein level of GLT-1 was down-regulated on day 14 following CCI and this phenomenon was reversed by fourteen days treatment of CLAV at the low doses of 12.5 and 25 mg/kg. Conclusion: These results suggest that CLAV might provide a new therapeutic strategy for neuropathic pain and its effect might be partially associated with the up-regulation of GLT-1. © 2019 Iran University of Medical Sciences. All rights reserved.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Hosseinzadeh, H.; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical SciencesIran; email: hosseinzadehh@mums.ac.ir
Uncontrolled Keywords: Chronic constriction injury Clavulanic Acid Glutamate Transporter 1
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 04:22
Last Modified: 21 Jun 2020 04:22
URI: http://eprints.mums.ac.ir/id/eprint/18249

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