Molecular characterization and antifungal susceptibilitytesting of Candida nivariensis from blood samples – an Iranian multicentre study and a review of the literature

Arastehfar, A. and Daneshnia, F. and Salehi, M. R. and Zarrinfar, H. and Khodavaisy, S. and Haas, P. J. and Roudbary, M. and Najafzadeh, M. J. and Zomorodian, K. and Charsizadeh, A. and Brouwer, C. and Pan, W. and Hagen, F. and Boekhout, T. (2019) Molecular characterization and antifungal susceptibilitytesting of Candida nivariensis from blood samples – an Iranian multicentre study and a review of the literature. Journal of Medical Microbiology, 68 (5). pp. 770-777.

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Molecular characterization and antifungal susceptibilitytesting of Candida nivariensis from blood samples – an Iranian multicentre study and a review of the literature.pdf

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Purpose. Identification of the emerging yeast species Candida nivariensis among presumptively identified Iranian Candida glabrata isolates. Methodology. Clinical C. glabrata species complex isolates from blood (n=100; 46.9), vaginal swabs (n=20; 9.4), bronchoalveolar lavage (n=10; 4.7) and sputum (n=12; 5.6) from 68 patients from Iran were investigated. Isolates were characterized by CHROMagar, multiplex PCRs, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), amplified fragment length polymorphism (AFLP) fingerprinting, internal transcribed spacer (ITS)/large subunit (LSU) rDNA and FKS1/FKS2 sequencing, and the European Committee on Antimicrobial Susceptibility Testing broth microdilution method. A comprehensive literature review was conducted and all the relevant clinical and microbiological data were collected. Results. Four C. nivariensis isolates were recovered from blood samples of three subjects and were all consistently identified by nine-plex PCR, Bruker MALDI-TOF MS, and LSU and ITS rDNA sequencing. AFLP genotyping clustered the isolates into two groups. Sequencing of the FKS1 and FKS2 hotspots showed no accountable amino acid substitutions. All isolates were susceptible to amphotericin B, fluconazole, itraconazole, posaconazole, voriconazole, anidulafungin and micafungin. Conclusion. In total, 4 out of 213 clinical C. glabrata species complex candidemia isolates were C. nivariensis. Improvement of the BioMerieux Vitek MS database is required to accurately identify C. nivariensis and it is advised to alternatively use CHROMagar and/or PCR-based techniques. As other species within the Nakaseomyces clade may cause infection and showed high MIC values for antifungals, inclusion of their spectra into the MALDI-TOF MS database seems relevant. Due to developing resistance to fluconazole and insufficient efficacy of caspofungin, the combination of catheter removal plus treatment with caspofungin, or voriconazole, or micafungin might be effective for patients. © 2019, Microbiology Society. All rights reserved.

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 CODEN: JMMIA Correspondence Address: Pan, W.; Department of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Institute of Medical Mycology, Shanghai Changzheng Hospital, Second Military Medical UniversityChina
Uncontrolled Keywords: Candida glabrata complex Candida nivariensis MALDI-TOF MS Multi-plex PCR Multicenter study Ribosomal DNA sequencing amikacin amphotericin B amphotericin B deoxycholate amphotericin B lipid complex anidulafungin antifungal agent boric acid caspofungin cefepime colistin fentanyl fluconazole flucytosine gentamicin imipenem internal transcribed spacer itraconazole meropenem micafungin miconazole nystatin posaconazole ribosome DNA rifampicin vancomycin voriconazole spacer DNA Acinetobacter infection adolescent aged amino acid substitution amplified fragment length polymorphism antifungal susceptibility antifungal therapy Article blood sampling Candida Candida glabrata candidiasis debridement drug substitution drug withdrawal female follow up heterozygote homozygote human lung lavage major clinical study male matrix assisted laser desorption ionization time of flight mass spectrometry minimum inhibitory concentration multiplex polymerase chain reaction nonhuman pain priority journal Pseudomonas aeruginosa skin graft blood candidemia case report clinical trial drug effect fatality genetics genotype Iran isolation and purification matrix-assisted laser desorption-ionization mass spectrometry microbial sensitivity test microbiology middle aged polymerase chain reaction vagina Amplified Fragment Length Polymorphism Analysis Antifungal Agents Bronchoalveolar Lavage DNA, Intergenic Fatal Outcome Humans Microbial Sensitivity Tests Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 20 Jun 2020 09:09
Last Modified: 20 Jun 2020 09:09

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