Effect of captopril on brain oxidative damage in pentylenetetrazole-induced seizures in mice

Asgharzadeh, F. and Hosseini, M. and Bargi, R. and Soukhtanloo, M. and Beheshti, F. and Mohammady, Z. and Anaeigoudari, A. (2019) Effect of captopril on brain oxidative damage in pentylenetetrazole-induced seizures in mice. Pharmaceutical Sciences, 25 (3). pp. 221-226.

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Background: Frequent seizure is followed by overproduction of free radicals and brain oxidative stress. Renin angiotensin system (RAS) has some effects on central nervous system. We designed this research to challenge the effect of captopril as an angiotensin converting enzyme (ACE) inhibitor against brain oxidative stress in pentylenetetrazole (PTZ)-induced seizures in mice. Methods: The groups were including (1) Control (saline); (2) PTZ (100 mg/kg, i.p.), (3-5) PTZ-captopril (Capto) that received three doses of Capto 10, 50 and 100 mg/kg 30 min before PTZ injection. Latency time in the onset minimal clonic seizures (MCS) and generalized tonic-clonic seizures (GTCS) were recorded. The level of malondialdehyde (MDA) and total thiol, as well as superoxide dismutase (SOD) and catalase (CAT) activity in the hippocampus and cortex were measured. Results: All doses of captopril postponed the onset of MCS and GTCS. Accumulation of MDA in the brain tissues of PTZ group was higher than control group, while total thiol content and CAT activity were lower. Pretreatment with captopril (100 mg/kg) diminished MDA concentration compared with PTZ group. Captopril (50 and 100 mg/kg) also increased the level of total thiol groups versus PTZ group. Captopril injection (50 and 100 mg/kg) elevated the activity of SOD and CAT in the brain tissues. In addition captopril administration diminished mortality rate caused by PTZ. Conclusion: Findings demonstrated that convulsions caused by PTZ were followed by oxidative stress status in the brain tissues. Pretreatment with captopril attenuated the effect of PTZ on brain tissue oxidative damage. ©2019 The Authors.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Asgharzadeh, F.; Psychiatry and Behavioral Sciences Research Center, Division of Neurocognitive Sciences, Mashhad University of Medical SciencesIran; email: Anaeiga317@gmail.com
Uncontrolled Keywords: Captopril Mice Oxidative stress Pentylenetetrazole Seizures catalase malonaldehyde pentetrazole superoxide dismutase animal experiment animal tissue Article brain cortex cerebral cortical tissue clonic seizure controlled study drug effect enzyme activity hippocampal tissue hippocampus latent period male minimal clonic seizure mortality rate mouse nonhuman pentylenetetrazole-induced seizure study design tonic clonic seizure
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 20 Jun 2020 09:25
Last Modified: 20 Jun 2020 09:25
URI: http://eprints.mums.ac.ir/id/eprint/18263

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