Ectopic expression of miRNA-21 and miRNA-205 in non-small cell lung cancer

Azimi, S. A. and Nia, H. R. S. and Jarrahi, A. M. and Jamaati, H. R. and Dizaji, M. K. and Dargahi, H. and Bahrami, N. and Pasdar, A. and Khosravi, A. and Bahrami, N. and Daghighi, S. and Mohamadnia, A. (2019) Ectopic expression of miRNA-21 and miRNA-205 in non-small cell lung cancer. International Journal of Cancer Management, 12 (1).

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Abstract

Background: Lung cancer is the leading cause of death, related to cancer in both men and women. MicroRNAs (miRNAs) play key roles in regulating the genes that are responsible for apoptosis, and regulation of gene expression. MicroRNA expression is deregulated in cancer. Many efforts have been made to detect cancer biomarkers. Extracellular miRNAs, which are directly released from tumors, can be detected in circulating blood. Objectives: The aim of this study was to examine the expression of miRNA-205 and-21 in lung cancer patients relative to healthy controls. Methods: This cross sectional study was performed in 50 healthy blood sample controls and 50 non-small cell lung cancer (NSCLC) blood samples. The expression of two miRNAs (miR-21 and miR-205) in peripheral blood mononuclear cells (PBMCs) was evaluated, using real-time polymerase chain reaction (real-time PCR) techniques. Statistical tests were carried out via the Mann-Whitney test. Results: The miR-205 levels of expression in the patients with lung cancer significantly increased in comparison to healthy controls (6.8 ± 0.42 and 1.2 ± 0.19, respectively), (P = 0.014). The expression of miR-21 in lung cancer (5.2 ± 0.52) significantly increased compared to the control group (1.6 ± 0.14) (P < 0.001). Conclusions: Based on our findings, lung cancer can enhance the ectopic expression of several miRNAs, which, in turn, play key roles in impaired apoptosis and further cancerous progression. © 2019, Author(s).

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Mohamadnia, A.; Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical SciencesIran; email: mohamadnia.ar@gmail.com
Uncontrolled Keywords: Expression MiR-205 MiR-21 Non-Small Cell Lung Cancer microRNA microRNA 205 microRNA 21 adult aged Article clinical article controlled study cross-sectional study ectopic expression female follow up histopathology human human cell human tissue male middle aged non small cell lung cancer peripheral blood mononuclear cell protein expression real time polymerase chain reaction reverse transcription polymerase chain reaction RNA extraction
Subjects: QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 05:00
Last Modified: 21 Jun 2020 05:00
URI: http://eprints.mums.ac.ir/id/eprint/18269

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