Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum leaves and its effect on lung pathological changes in an ovalbumin-induced rat model of asthma

Eftekhar, N. and Moghimi, A. and Mohammadian Roshan, N. and Saadat, S. and Boskabady, M. H. (2019) Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum leaves and its effect on lung pathological changes in an ovalbumin-induced rat model of asthma. BMC Complementary and Alternative Medicine, 19 (1).

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Abstract

Background: Ocimum species (Lamiaceae) has been traditionally used for treatment of upper respiratory tract infections, bronchitis, coughs, sore throat, and wound healing. The Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum (O. basilicum) leaves was examined in ovalbumin sensitized animals. Methods: Wistar rats were divided to six groups; non-sensitized, sensitized to ovalbumin, sensitized and treated with dexamethasone (1.25 μg/mL), and O. basilicum extract (0.75, 1.50 and 3.00 mg/mL) in drinking water for 21 days. The levels of interleukin 4 (IL-4), interferon gamma (IFN-γ), IFN-γ/IL-4 ratio, immunoglobulin E (IgE), phospholipase A2 (PLA2) and total protein (TP) in BALF, and lung pathological changes were examined. Results: A significant increase in IL-4, IgE, PLA2 and TP levels, all lung pathological indices as well as significant decrease in IFN-γ/IL-4 ratio was seen in the asthmatic compared to the control rats (P < 0.05 to P < 0.001). Treatment with O. basilicum extract resulted in decreased IL-4, IgE, PLA2 and TP levels, but increased IFN-γ/IL-4 ratio compared to untreated sensitized rats (P < 0.01 to P < 0.001). The plant significantly improved the pathological changes of sensitized rats (P < 0.05 to P < 0.01). The improvement effects of higher concentrations of the O. basilicum extract were significantly more than those of dexamethasone (P < 0.05 to P < 0.001). Conclusion: The improvement effects of O. basilicum on pathological changes, immunological and inflammatory markers in sensitized rats comparable or even more potent than dexamethasone suggests the therapeutic potential of the plant in asthma. © 2019 The Author(s).

Item Type: Article
Additional Information: Export Date: 16 February 2020 CODEN: BCAMC Correspondence Address: Boskabady, M.H.; Neurogenic Inflammation Research Center, Mashhad University of Medical SciencesIran; email: boskabadymh@mums.ac.ir
Uncontrolled Keywords: Asthma Inflammatory mediators Ocimum basilicum, immunological mediators dexamethasone gamma interferon immunoglobulin E interleukin 4 Ocimum basilicum extract phospholipase A2 protein antiinflammatory agent cytokine ovalbumin plant extract animal cell animal experiment animal model animal tissue antiinflammatory activity Article basil bronchoalveolar lavage fluid controlled study drug effect drug efficacy experimental asthma histology immunomodulation male nonhuman ovalbumin-induced asthma plant leaf rat rat model Th1 Th2 balance Wistar rat animal chemistry disease model lung metabolism pathology Animals Anti-Inflammatory Agents Cytokines Disease Models, Animal Ocimum basilicum Plant Extracts Plant Leaves Rats Rats, Wistar
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 05:08
Last Modified: 21 Jun 2020 05:08
URI: http://eprints.mums.ac.ir/id/eprint/18314

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