MCF-7 and its multidrug resistant variant MCF-7/ADR overcome TNF cytotoxicity through prevention of reactive oxygen species accumulation

Ghandadi, M. and Behravan, J. and Biabani, S. and Abaspor, S. and Mosaffa, F. (2019) MCF-7 and its multidrug resistant variant MCF-7/ADR overcome TNF cytotoxicity through prevention of reactive oxygen species accumulation. Pharmaceutical Sciences, 25 (2). pp. 118-123.

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Abstract

Background: Signal transduction of numerous cytokines and growth factors are mediated by reactive oxygen species (ROS). Tumor necrosis factor-α (TNF-α) have stimulated accumulation of ROS in various in vitro studies. MCF-7 and its Adriamycin resistant variant MCF-7/ADR are resistant against TNF-α cytotoxicity. Role of ROS in the resistance of MCF-7 and MCF-7/ADR cells was investigated. Methods: ROS accumulation and viability in MCF-7 and MCF-7/ADR after TNF-α exposure was evaluated using 2',7'-dichlorofluorescein diacetate (DCFH-DA) as a fluorescent probe and 3-4, 5-dimethylthiazol-2-yl-2, 5 diphenyltetrazolium bromide (MTT) cytotoxicity assay respectively. Results: ROS level did not change significantly after TNF-α exposure. Induction of ROS accumulation along with TNF-α treatment sensitized these cells to TNF-α toxicity. Conclusion: It can be concluded that lack of ROS accumulation following TNF-α exposure is involved at least by part in the resistance of MCF-7 and its drug resistant derivative MCF-7/ADR cells to TNF-α cytotoxicity. © 2019 The Authors.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Mosaffa, F.; Biotechnology Research Center, Mashhad University of Medical SciencesIran; email: mosaffaf@mums.ac.ir
Uncontrolled Keywords: Breast cancer MCF-7 Multidrug resistance ROS TNF-α catalase dichlorofluorescein fluorescent dye glutathione peroxidase glutathione reductase reactive oxygen metabolite superoxide dismutase tumor necrosis factor Article bioaccumulation cell viability controlled study cytotoxicity enzyme activity MCF-7 cell line MTT assay NCI-ADR-RES cell line signal transduction
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 05:15
Last Modified: 21 Jun 2020 05:15
URI: http://eprints.mums.ac.ir/id/eprint/18350

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