Stimulation of Osteogenic Differentiation of Induced Pluripotent Stem Cells (iPSCs) Using Bioactive Glasses: An in vitro Study

Kargozar, S. and Lotfibakhshaeish, N. and Ebrahimi-Barough, S. and Nazari, B. and Hill, R. G. (2019) Stimulation of Osteogenic Differentiation of Induced Pluripotent Stem Cells (iPSCs) Using Bioactive Glasses: An in vitro Study. Frontiers in Bioengineering and Biotechnology, 7.

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Abstract

Selection and use of an optimal cell source for bone tissue engineering (BTE) remain a challenging issue; the invention of induced pluripotent stem cells (iPSCs) have created new hopes on this regard. At the present study, we attempted to show the usability of iPSCs in combination with bioactive glasses (BGs) for bone regeneration applications. For this aim, iPSCs were cultured and incubated with the strontium and cobalt-containing BGs for different intervals (1, 5, and 7 days). The cell cytotoxicity and attachment were assessed using MTT assay and scanning electron microscopy (SEM), respectively. Moreover, the osteogenic differentiation of iPSCs seeded onto the glasses was evaluated using alkaline phosphatase (ALP) activity assay and real-time PCR. The obtained results clarified that although the cell viability is decreased during a 7 day period, the iPSCs could adhere and expand onto the BGs particles and over-express the osteogenic markers, including osteocalcin, osteonectin, and Runx2. Based on the data, we conclude that iPSCs in a combination of BGs can be considered as a potential candidate for BTE strategies. © Copyright © 2019 Kargozar, Lotfibakhshaeish, Ebrahimi-Barough, Nazari and Hill.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Lotfibakhshaeish, N.; Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical SciencesIran; email: n.lotfiba@gmail.com
Uncontrolled Keywords: bioactive glass bone tissue engineering in vitro induced pluripotent stem cells osteogenesis Bone Cytology Phosphatases Polymerase chain reaction Scanning electron microscopy Stem cells Tissue Tissue engineering Alkaline phosphatase activity Bone regeneration Cell viability In-vitro Osteogenic differentiation Cell engineering
Subjects: QT physiology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 06:46
Last Modified: 21 Jun 2020 06:46
URI: http://eprints.mums.ac.ir/id/eprint/18445

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