Reducing effect of insulin resistance on alpha-synuclein gene expression in skeletal muscle

Khoshi, A. and Goodarzi, G. and Mohammadi, R. and Arezumand, R. and Moghbeli, M. and Najariyan, M. (2019) Reducing effect of insulin resistance on alpha-synuclein gene expression in skeletal muscle. Diabetology and Metabolic Syndrome, 11 (1).

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Abstract

Background: Alpha-synuclein (SNCA) as the presynaptic protein is expressed in different tissues and prevents insulin-resistance (IR) through increasing glucose-uptake by adipocytes and muscles. However, the effect of insulin metabolism on SNCA expression has scarcely elucidated. In present study we assessed the probable effect of insulin resistance on SNCA expression in muscle C2C12 cells and also skeletal muscle tissues of type 2 diabetic mice. Materials and methods: Sixteen male C57BL/6 mice were divided into two experimental groups, including control and type 2 diabetic mice with IR (induced by high-fat diet + low-dose streptozotocin). The animals of the study involved the measurements of fasting blood glucose, oral-glucose-tolerance-test, as well as fasting plasma insulin. Moreover, insulin-resistant and insulin-sensitive muscle C2C12 cells were prepared. The insulin-resistance was confirmed by the glucose-uptake assay. Comparative quantitative real time PCR was used to assess the SNCA expression. Results: The obtained results have showed a significant ~ 27 decrease in SNCA expression level in muscle tissue of diabetic mice (P = 0.022). Moreover, there was a significant change of SNCA expression in insulin-resistant C2C12 cells (P < 0.001). Conclusion: Type 2 diabetes due to insulin-resistance can decrease SNCA gene expression in muscles. In addition to the role of SNCA in cell susceptibility to insulin and glucose uptake, the SNCA expression can also be affected by insulin metabolism. © 2019 The Author(s).

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Khoshi, A.; Department of Clinical Biochemistry, School of Medicine, North Khorasan University of Medical Sciences, Arkan Roadway, Iran; email: ahkh83@gmail.com
Uncontrolled Keywords: Alpha-synuclein C2C12 muscle cells Insulin resistance Muscle tissue Type 2 diabetes alpha synuclein glucose insulin streptozocin alpha synuclein gene animal cell animal experiment animal model animal tissue Article bioassay C2C12 cell line C57BL 6 mouse controlled study gene expression glucose blood level glucose transport glucose uptake assay insulin blood level insulin metabolism lipid diet male mouse non insulin dependent diabetes mellitus nonhuman oral glucose tolerance test priority journal real time polymerase chain reaction skeletal muscle streptozotocin-induced diabetes mellitus
Subjects: WD Nutrition Disease and metabolic diseases
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 07:19
Last Modified: 21 Jun 2020 07:19
URI: http://eprints.mums.ac.ir/id/eprint/18473

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