Comparison the effect of ferutinin and 17β-estradiol on bone mineralization of developing zebrafish (Danio rerio) Larvae

Mirakabad, H. Z. and Farsi, M. and Shafaroudi, S. M. and Bagheri, A. and Iranshahi, M. and Moshtaghi, N. (2019) Comparison the effect of ferutinin and 17β-estradiol on bone mineralization of developing zebrafish (Danio rerio) Larvae. International Journal of Molecular Sciences, 20 (6).

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Abstract

There is an urgent need to develop novel drugs for osteoporosis which occurs due to estrogen deficiency. Phytoestrogens derived from medicinal plants would be the best alternative to chemical drugs with harmful side effects. The main purpose of the present study was to investigate the effect of ferutinin compared to 17β-estradiol (E2) on bone mineralization of zebrafish larvae. Regarding the lack of publications, the histology analysis was performed after exposure to E2 to find effective treatment on bone mineralization of developing zebrafish larvae. Then, the larvae were exposed to four concentrations of ferutinin at three time points to assess the mortality, the expression of some related genes and histology of the ceratohyal and hyomandibular of treated larvae. The RT-PCR result of the treatment groups demonstrated the similar expression pattern in the larvae which were exposed to 1.25 µg/mL of ferutinin and 2 µM of E2 at 2 dpf, which confirmed the result of histology analysis. In addition, RT-qPCR of high concentration of ferutinin and E2 demonstrated that bmp2a/b and esr1 were downregulated and upregulated when the larvae were exposed to 5 µg/mL of ferutinin and 10 µM of E2, respectively. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Farsi, M.; Department of Biotechnology and Plant Breeding, Ferdowsi University of MashhadIran; email: farsi@um.ac.ir
Uncontrolled Keywords: 17β-estradiol Chemical screening Ferutinin Histology LC50 Osteogenesis Real-time PCR RT-PCR Skeletal tissue Zebrafish bone morphogenetic protein 2 estradiol estrogen receptor estrogen receptor alpha estrogen related receptor alpha ribosome protein terpenoid derivative unclassified drug 4-oxy-6-(4-oxybezoyloxy)dauc-8,9-en benzoic acid bmp2b protein, zebrafish bridged bicyclo compounds cycloheptane derivative estrogen estrogen receptor 1, zebrafish sesquiterpene zebrafish protein animal experiment animal tissue Article bone defect bone density bone development bone metabolism bone mineralization bone regeneration cell differentiation Chironomidae chondrocyte comparative study controlled study dose response drug effect embryo exposure gene gene expression in situ hybridization induced pluripotent stem cell larva mortality nonhuman osteoblast osteoporosis phenotype real time polymerase chain reaction reverse transcription polymerase chain reaction RNA extraction RNA isolation screening stereomicroscopy toxicity zebra fish animal genetics metabolism Animals Benzoates Calcification, Physiologic Cycloheptanes Estrogens Sesquiterpenes Zebrafish Proteins
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 08:05
Last Modified: 21 Jun 2020 08:05
URI: http://eprints.mums.ac.ir/id/eprint/18504

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