Amelioration of renal and hepatic function, oxidative stress, inflammation and histopathologic damages by Malva sylvestris extract in gentamicin induced renal toxicity

Mohamadi Yarijani, Z. and Najafi, H. and Shackebaei, D. and Madani, S. H. and Modarresi, M. and Jassemi, S. V. (2019) Amelioration of renal and hepatic function, oxidative stress, inflammation and histopathologic damages by Malva sylvestris extract in gentamicin induced renal toxicity. Biomedicine and Pharmacotherapy, 112.

[img] Text
Amelioration of renal and hepatic function, oxidative stress, inflammation and histopathologic damages by Malva sylvestris extract in gentamicin induced renal toxicity.pdf

Download (2MB)

Abstract

Background: Gentamycin, contrary to its wide range of antimicrobial effects, has a high potential for nephrotoxicity, and renal injury can have effects on other organs such as the liver. Objectives: The aim of the present study was to assess the effects of hydro alcoholic Malva sylvestris(MS) extract on nephrotoxicity induced by gentamicin, and also its remote organ injury in the liver. Methods: Renal and hepatic functions were evaluated through measurement of creatinine, urea-nitrogen, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in plasma. Oxidative stress was assessed through measuring malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP) levels, and histopathologic injuries were evaluated using H & E stained sections. For evaluation of inflammation, TNF-α and ICAM-1 mRNA expression levels were measured in the renal tissue using Real-time PCR method. Results: Gentamicin resulted in an increase in the levels of creatinine, urea-nitrogen, AST, ALT, and ALP in the plasma, as well as an increase in TNF-α and ICAM-1 mRNA expression levels in the renal tissue, renal and hepatic histopathologic injuries and MDA level, and a decrease in FRAP. Administration of MS led to improvement in the function of kidney and liver, a decrease in the expression levels of proinflammatory factors, reduction of oxidative stress, and also a decrease in tissue injuries. Conclusion: MS extract can protect the kidney against toxic effects of gentamicin, and thus, the degree of harmful effects of nephrotoxicity on remote organs including the liver will be decreased. © 2019 The Authors

Item Type: Article
Additional Information: Cited By :6 Export Date: 16 February 2020 CODEN: BIPHE Correspondence Address: Najafi, H.; Medical Biology Research Center, Kermanshah University of Medical SciencesIran; email: houshang.najafi@gmail.com
Uncontrolled Keywords: Anthocyanins Gentamicin Inflammation Malva sylvestris Oxidative stress Remote organ injury alanine aminotransferase alkaline phosphatase alkaloid anthocyanin anthraquinone derivative antiinflammatory agent antioxidant aspartate aminotransferase coumarin derivative creatinine flavonoid intercellular adhesion molecule 1 malonaldehyde Malva sylvestris extract messenger RNA nitrogen plant extract saponin sterol tannin derivative triterpenoid tumor necrosis factor unclassified drug urea alanine aminotransferase blood level alkaline phosphatase blood level animal cell animal experiment animal model animal tissue antiinflammatory activity antioxidant activity Article aspartate aminotransferase blood level comparative study controlled study creatinine blood level histopathology kidney function kidney tissue lipid peroxidation liver function liver tissue liver toxicity male Malva mRNA expression level nephrotoxicity nonhuman phytochemistry priority journal rat real time polymerase chain reaction urea nitrogen blood level animal chemistry dose response drug effect immunology isolation and purification kidney kidney disease kidney function test liver liver disease liver function test pathology Wistar rat Animals Dose-Response Relationship, Drug Gentamicins Kidney Diseases Kidney Function Tests Liver Diseases Liver Function Tests Plant Extracts Rats, Wistar
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 09:12
Last Modified: 21 Jun 2020 09:12
URI: http://eprints.mums.ac.ir/id/eprint/18520

Actions (login required)

View Item View Item