Contrast and spatial frequency modulation for diagnosis of amblyopia: An electrophysiological approach

Mohammadi, A. and Hashemi, H. and Mirzajani, A. and Yekta, A. and jafarzadehpur, E. and Valadkhan, M. and Khabazkhoob, M. (2019) Contrast and spatial frequency modulation for diagnosis of amblyopia: An electrophysiological approach. Journal of Current Ophthalmology, 31 (1). pp. 72-79.

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Purpose: To evaluate the diagnostic value of visual evoked potentials (VEPs) and to find out which test setting has the most sensitivity and specificity for amblyopia diagnosis. Methods: Thirty-three adult anisometropic amblyopes were intended in this study and were tested for visual evoked potentials with different stimulus conditions including three spatial frequencies 1, 2, and 4-cycles-per-degree (cpd) at four contrast levels (100, 50, 25, and 5%). We also tested psychophysical contrast sensitivity and compared the results with electrophysiological ones. We plotted Receiver Operating Characteristic (ROC) curve for each VEP recording and psychophysical contrast sensitivity to evaluate the area under the curve, sensitivity, specificity, and cut-point value of each test stimulus for detecting amblyopic eyes. Results: Thirty-three amblyopic and 33 non-amblyopic eyes were examined for psychophysical contrast sensitivity and VEPs. Area under the ROC curve (AURC) findings showed that VEP with different stimulus settings can significantly detect amblyopic eyes, as well as psychophysical contrast sensitivity test. We found that P100 amplitudes had the largest AURC in response to stimuli of 2-cpd spatial frequency at 50 (P < 0.001) and 25% (P < 0.001) contrast levels, respectively. Cut-off amplitudes for these stimuli were 8.65 and 4.50 μV, which had a sensitivity of 0.758 and 0.697 and a specificity of 0.788 and 0.848, respectively. The sensitivity and specificity of VEP P100 amplitude in response to the stimuli with 2 cpd spatial frequency and 50 and 25% contrast were greater than the findings obtained from psychophysical contrast sensitivity test. Conclusion: According to our findings, assessment of VEP amplitudes in response to stimuli of 2-cpd spatial frequency at 50 and 25% contrast levels can best detect amblyopia with highest sensitivity and specificity and thus, are the protocols of choice for detection of amblyopic eyes. © 2018 Iranian Society of Ophthalmology

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: jafarzadehpur, E.; Department of Optometry, School of Rehabilitation Sciences, Iran University of Medical SciencesIran; email:
Uncontrolled Keywords: Amblyopia Contrast sensitivity Visual evoked potentials adolescent adult Article clinical article diagnostic value electrophysiology frequency modulation human psychophysiology sensitivity and specificity spatial analysis visual acuity visual evoked potential Youden index
Subjects: WW Ophthalmology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 09:10
Last Modified: 21 Jun 2020 09:10

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