Investigating the vinblastine induced-chromosomal abnormality in the already gamma irradiated L929 cell line using micronucleus assay in cytokinesis blocked binucleated cells

Mohammadi, Z. and Haddad, F. and Matin, M. M. and Soleymanifard, S. (2019) Investigating the vinblastine induced-chromosomal abnormality in the already gamma irradiated L929 cell line using micronucleus assay in cytokinesis blocked binucleated cells. Asian Pacific Journal of Cancer Prevention, 20 (4). pp. 1045-1050.

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Investigating the vinblastine induced-chromosomal abnormality in the already gamma irradiated L929 cell line using micronucleus assay in cytokinesis blocked binucleated cells.pdf

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Abstract

Objectives: Vast number of studies show the relationship between aneuploidy and cancer. Ionizing radiation in addition to induce all kinds of damages to the cells and structure of chromosomes, is also able to induce aneuploidy through direct damages to chromosome division apparatus. Also irradiation of the cells induces mutations in several genes which might be involved in cell division fidelity and play a role in reversing the effect of aneugens. Therefore, irradiation of cells and tissues might produce sensitivity to agents with aneugenic capability in irradiated cells. Methods: To investigate the persistent genomic effect of ionizing irradiation on chromosomal instability, L929 cells were gamma irradiated with the dose of 2 Gy. Cells were left to recover from the harmful effect of irradiation. They were treated with low dose of vinblastine (0.5 ng.ml-1) 72h post-gamma irradiation. Finally, the induced chromosomal abnormalities were scored using micronucleus assay in cytokinesis-blocked binucleated cells (MnBi). Results: Irradiation-recovered L929 cells treated with vinblastine showed a statistically higher frequency of MnBi compared to non-irradiated and vinblastine treated cells. Conclusion: The results indicate that gamma irradiation, in addition to direct induction of chromosomal damages, is also able to create persisting genomic sensitivity in the cells to chromosomal instability, which is detectable when exposed to the second stimulus. © 2019 Asian Pacific Organization for Cancer Prevention.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Haddad, F.; Department of Biology, Faculty of Sciences, Cell and Molecular Biotechnology Research Group, Ferdowsi University of MashhadBrazil; email: haddad@um.ac.ir
Uncontrolled Keywords: Gamma irradiation L929 cell Micronucleus assay Vinblastine antineoplastic agent cell culture cell division cell nucleus chromosome aberration cytokinesis drug effect fibroblast gamma radiation genetics human micronucleus test pathology radiation response Antineoplastic Agents, Phytogenic Cells, Cultured Chromosome Aberrations Fibroblasts Gamma Rays Humans Micronucleus Tests
Subjects: QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 09:10
Last Modified: 21 Jun 2020 09:10
URI: http://eprints.mums.ac.ir/id/eprint/18523

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