Overexpression of MicroRNA-148b-3p stimulates osteogenesis of human bone marrow-derived mesenchymal stem cells: The role of MicroRNA-148b-3p in osteogenesis

Mollazadeh, S. and Fazly Bazzaz, B. S. and Neshati, V. and De Vries, A. A. F. and Naderi-Meshkin, H. and Mojarad, M. and Mirahmadi, M. and Neshati, Z. and Kerachian, M. A. (2019) Overexpression of MicroRNA-148b-3p stimulates osteogenesis of human bone marrow-derived mesenchymal stem cells: The role of MicroRNA-148b-3p in osteogenesis. BMC Medical Genetics, 20 (1).

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Overexpression of MicroRNA-148b-3p stimulates osteogenesis of human bone marrow-derived mesenchymal stem cells The role of MicroRNA-148b-3p in osteogenesis.pdf

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Abstract

Background: Mesenchymal stem cells (MSCs) are attractive choices in regenerative medicine and can be genetically modified to obtain better results in therapeutics. Bone development and metabolism are controlled by various factors including microRNAs (miRs) interference, which are small non-coding endogenous RNAs. Methods: In the current study, the effects of forced miR-148b expression was evaluated on osteogenic activity. Human bone marrow-derived mesenchymal stem cells (BM-MSCs) were transduced with bicistronic lentiviral vector encoding hsa-miR-148b-3p or -5p and the enhanced green fluorescent protein. Fourteen days post-transduction, immunostaining as well as Western blotting were used to analyze osteogenesis. Results: Overexpression of miR-148b-3p increased the osteogenic differentiation of human BM-MSCs as demonstrated by anenhancement of mineralized nodular formation and an increase in the levels of osteoblastic differentiation biomarkers, alkaline phosphatase and collagen type I. Conclusions: Since lentivirally overexpressed miR-148b-3p increased osteogenic differentiation capability of BM-MSCs, this miR could be applied as a therapeutic modulator to optimize bone function. © 2019 The Author(s).

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 CODEN: BMGMA Correspondence Address: Kerachian, M.A.; Medical Genetics Research Center, Mashhad University of Medical SciencesIran; email: amin.kerachian@mail.mcgill.ca
Uncontrolled Keywords: Lentivirus Mesenchymal stem cells MicroRNA-148b Osteogenesis alkaline phosphatase collagen type 1 enhanced green fluorescent protein microRNA microRNA 148b 3p microRNA 148b 5p unclassified drug biological marker MIRN148 microRNA, human Article bone development bone marrow biopsy bone marrow derived mesenchymal stem cell cell differentiation controlled study fluorescence microscopy gene function gene overexpression human human cell human tissue immunohistochemistry immunoreactivity incubation time regulatory mechanism Western blotting bone marrow cytology gene vector genetic transduction genetics growth, development and aging HEK293 cell line mesenchymal stem cell metabolism nucleotide sequence pathology Base Sequence Biomarkers Collagen Type I Genetic Vectors HEK293 Cells Humans MicroRNAs Transduction, Genetic
Subjects: QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 09:20
Last Modified: 21 Jun 2020 09:20
URI: http://eprints.mums.ac.ir/id/eprint/18532

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