Unexpected inflammation in the sympathetic ganglia in thromboangiitis obliterans: More likely sterile or infectious induced inflammation?

Mousazadeh, B. and Sharebiani, H. and Taheri, H. and Valizedeh, N. and Fazeli, B. (2019) Unexpected inflammation in the sympathetic ganglia in thromboangiitis obliterans: More likely sterile or infectious induced inflammation? Clinical and Molecular Allergy, 17 (1).

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Abstract

Introduction: The aim of this study was to determine if the inflammation of the sympathetic ganglia (SG) in thromboangiitis obliterans (TAO) is induced by an infectious pathogen inside or if it is a reactive sterile inflammation. Methods: For the purpose of this study, the gene expression of high-mobility group box 1 (HMGB1), toll-like receptor 4 (TLR4), toll-like receptor 9 (TLR9), and the receptor for advanced glycation end-products (RAGE) were evaluated on the complementary DNA (cDNA) of the SG tissues of 24 TAO patients and two controls with hyperhidrosis by real-time polymerase chain reaction (PCR) and analysed by the Pfaffl method. Results: The gene expression of HMGB1 and TLR9 increased by about 25- and 2-fold changes in the SG of the TAO patients, respectively. However, there was no change in the gene expression of TLR4 or RAGE. Conclusion: It appears that the inflammation in the SG of TAO patients is more likely a sterile inflammation, and its trigger may be mitochondrial DNA (mtDNA). Cadmium in cigarettes could be responsible for the induction of mtDNA release to the cell cytoplasm. In addition, the high expression of HMGB1 may play a role in the pathogenesis of TAO and may be responsible for both clinical manifestation of the disease and the imaging findings. Moreover, HMGB1 may be a target for treatment protocols for TAO. Further studies are highly recommended. © 2019 The Author(s).

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Fazeli, B.; Immunology Research Center, Inflammation and Inflammatory Diseases Division, School of Medicine, Mashhad University of Medical SciencesIran; email: bahar.fazeli@gmail.com
Uncontrolled Keywords: Buerger's disease HMGB1 Neurogenic inflammation RAGE Sympathectomy Sympathetic ganglia Thromboangiitis obliterans TLR4 TLR9 advanced glycation end product receptor complementary DNA high mobility group B1 protein toll like receptor 4 toll like receptor 9 adult Article Buerger disease clinical article controlled study gene expression human hyperhidrosis inflammation male middle aged protein folding real time polymerase chain reaction
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 09:22
Last Modified: 21 Jun 2020 09:22
URI: http://eprints.mums.ac.ir/id/eprint/18551

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