Effects of Quercetin-loaded nanoparticles on MCF-7 human breast cancer cells

Niazvand, F. and Orazizadeh, M. and Khorsandi, L. and Abbaspour, M. and Mansouri, E. and Khodadadi, A. (2019) Effects of Quercetin-loaded nanoparticles on MCF-7 human breast cancer cells. Medicina (Lithuania), 55 (4).

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Background and objectives: Previous studies have shown anti-tumor activity of quercetin (QT). However, the low bioavailability of QT has restricted its use. This study aimed to assess the toxic effect of QT encapsulated in solid lipid nanoparticles (QT-SLNs) on the growth of MCF-7 human breast cancer cells.Materials and Methods: MCF-7 and MCF-10A (non-tumorigenic cell line) cell lines treated with 25 µmol/mL of QT or QT-SLNs for 48 h. Cell viability, colony formation, oxidative stress, and apoptosis were evaluated to determine the toxic effects of the QT-SLNs. Results: The QT-SLNs with appropriate characteristics (particle size of 85.5 nm, a zeta potential of −22.5 and encapsulation efficiency of 97.6) were prepared. The QT-SLNs showed sustained QT release until 48 h. Cytotoxicity assessments indicated that QT-SLNs inhibited MCF-7 cells growth with a low IC 50 (50 inhibitory concentration) value, compared to the free QT. QT-SLNs induced a significant decrease in the viability and proliferation of MCF-7 cells, compared to the free QT. QT-SLN significantly increased reactive oxygen species (ROS) level and MDA contents and significantly decreased antioxidant enzyme activity in the MCF-7 cells. Following QT-SLNs treatment, the expression of the Bcl-2 protein significantly decreased, whereas Bx expression showed a significant increase in comparison with free QT-treated cells. Furthermore, The QT-SLNs significantly increased apoptotic and necrotic indexes in MCF-7 cells. Viability, proliferation, oxidative stress and apoptosis of MCF-10A cells were not affected by QT or QT-SLNs. Conclusions: According to the results of this study, SLN significantly enhanced the toxic effect of QT against human breast cancer cells. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 Correspondence Address: Khorsandi, L.; Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical SciencesIran; email: khorsandicmrc@yahoo.com
Uncontrolled Keywords: Apoptosis Breast cancer Nanomedicine Quercetin Solid lipid nanoparticles Toxicity antioxidant catalase malonaldehyde nanocapsule reactive oxygen metabolite superoxide dismutase breast tumor cell proliferation cell survival drug delivery system drug effect female human MCF-7 cell line oxidative stress particle size procedures treatment outcome Antioxidants Breast Neoplasms Drug Delivery Systems Humans Malondialdehyde MCF-7 Cells Nanocapsules Reactive Oxygen Species
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 09:30
Last Modified: 21 Jun 2020 09:30
URI: http://eprints.mums.ac.ir/id/eprint/18572

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