Co-administration and evaluation of immune responses of three DNA vaccines encoding immunogenic antigens from Mycobacterium tuberculosis

Peeridogaheh, H. and Teimourpour, R. and Habibzadeh, S. and Mohammadshahi, J. and Gholoobi, A. and Teimourpour, A. and Meshkat, Z. (2019) Co-administration and evaluation of immune responses of three DNA vaccines encoding immunogenic antigens from Mycobacterium tuberculosis. Archives of Clinical Infectious Diseases, 14 (3).

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Abstract

Background: Ineffectiveness of BCG vaccine in controlling tuberculosis (TB) and co-infection of TB and HIV have turned TB into a serious global threat. Therefore, the development of an alternative vaccine to BCG and/or antimycobacterial drugs is an urgent need. Here, three chimeric DNA constructs consisting of Mtb32C-HBHA, Ag85a-Tb10.4, and Ag85a-cfp10 made in our previous studies were co-administered to BALB/c mice to evaluate their immune responses using a prime-boost regimen in which the animals were first immunized with BCG and then administered with DNA vaccines. Methods: In order to evaluate the immunogenicity of three DNA constructs, the levels of several immunomodulatory cytokines were measured in vaccinated mice. Thirty female BALB/c mice were divided into the following groups (n = 10): control (receiving pcDNA 3.1+ intramuscularly), vaccine (receiving recombinant vectors intramuscularly), and vaccine-BCG (receiving BCG subcutaneously followed by recombinant vectors intramuscularly). Results: The levels of IL-4, IL-12, TGF-β, IFN-γ, and IL-10 were higher in the immunized groups than in the control group (P < 0.05). Besides, the levels of IL-12 and IFN-γ were much higher in the BCG-vaccine group than in the vaccine alone group. In the case of IFN-γ, a significant difference was observed between the vaccine and BCG-vaccine groups at P < 0.001 while in the case of IL-12, the difference was significant at P < 0.05. However, in the case of IL-10, IL-4, and TGF-β, the differences between the vaccine and BCGvaccine groups were not significant (P > 0.05). Conclusions: Our results proved that using a chimeric DNA vaccine as a booster in the prime-boost strategy could significantly enhance the efficacy of BCG. This study suggests that the use of such DNA vaccines encoding mycobacterial immunogenic antigens as boosters enhances the efficacy of BCG. © 2019, Archives of Clinical Infectious Diseases.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Meshkat, Z.; Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, P.O. Box: 9196773117, Iran; email: meshkatz@mums.ac.ir
Uncontrolled Keywords: BALB/c mice BCG DNA HIV Vaccine bacterial antigen BCG vaccine DNA vaccine gamma interferon interleukin 10 interleukin 12 interleukin 4 transforming growth factor beta animal cell animal experiment animal tissue Article controlled study cytokine production enzyme linked immunosorbent assay female immune response immunization immunogenicity immunomodulation mouse Mycobacterium tuberculosis nonhuman tuberculosis
Subjects: QW Microbiology and Immunology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 21 Jun 2020 09:31
Last Modified: 21 Jun 2020 09:31
URI: http://eprints.mums.ac.ir/id/eprint/18584

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