Anti-Inflammatory and Anti-Oxidant Activity of Portulaca oleracea Extract on LPS-Induced Rat Lung Injury

Rahimi, V. B. and Rakhshandeh, H. and Raucci, F. and Buono, B. and Shirazinia, R. and Kermani, A. S. and Maione, F. and Mascolo, N. and Askari, V. R. (2019) Anti-Inflammatory and Anti-Oxidant Activity of Portulaca oleracea Extract on LPS-Induced Rat Lung Injury. Molecules, 24 (1).

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Abstract

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are classified as two lung complications arising from various conditions such as sepsis, trauma, and lung inflammation. Previous studies have shown that the extract of the leaves of Portulaca oleracea (PO) possesses anti-inflammatory and anti-oxidant activities. In the present study, the effects of PO (50–200 mg/kg) and dexamethasone (Dexa; 1.5 mg/kg) on lipopolysaccharide (LPS)-induced ALI were investigated. Subsequentially, the lung wet/dry ratio; white blood cells (WBC); levels of nitric oxide (NO); myeloperoxidase (MPO); malondialdehyde (MDA); thiol groups formation; super oxide dismutase (SOD) and catalase (CAT) activities; and levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6, IL-10, prostaglandin E2 (PGE2), and transforming growth factor (TGF)-β in the broncho alveolar lavage fluid (BALF) were evaluated in order to demonstrate the anti-oxidant and anti-inflammatory activity of PO. Our results show that PO suppresses lung inflammation by the reduction of IL-β, IL-6, TNF-α, PGE2, and TGF-β, as well as by the increase of IL-10 levels. We also found that PO improves the level of WBC, MPO, and MDA, as well as thiol group formation and SOD and CAT activities, compared with the LPS group. The results of our investigation also show that PO significantly decreased the lung wet/dry ratio as an index of interstitial edema. Taken together, our findings reveal that PO extract dose-dependently displays anti-oxidant and anti-inflammatory activity against LPS-induced rat ALI, paving the way for rational use of PO as a protective agent against lung-related inflammatory disease. © 2019 by the authors.

Item Type: Article
Additional Information: Cited By :7 Export Date: 16 February 2020 CODEN: MOLEF Correspondence Address: Maione, F.; Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, Italy; email: francesco.maione@unina.it
Uncontrolled Keywords: Acute lung injury Inflammation Portulaca oleracea antiinflammatory agent antioxidant autacoid biological marker cytokine lipopolysaccharide plant extract animal bronchoalveolar lavage fluid chemistry disease model male metabolism organ size pathology Portulaca rat Animals Anti-Inflammatory Agents Antioxidants Biomarkers Cytokines Disease Models, Animal Inflammation Mediators Lipopolysaccharides Plant Extracts Rats
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 27 Jun 2020 05:28
Last Modified: 27 Jun 2020 05:28
URI: http://eprints.mums.ac.ir/id/eprint/18598

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