Synergistic effects of deuterium depleted water and Mentha longifolia L. essential oils on sepsis-induced liver injuries through regulation of cyclooxygenase-2

Rasooli, A. and Fatemi, F. and Hajihosseini, R. and Vaziri, A. and Akbarzadeh, K. and Mohammadi Malayeri, M. R. and Dini, S. and Foroutanrad, M. (2019) Synergistic effects of deuterium depleted water and Mentha longifolia L. essential oils on sepsis-induced liver injuries through regulation of cyclooxygenase-2. Pharmaceutical Biology, 57 (1). pp. 125-132.

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Context:Mentha longifolia L. (Lamiaceae), a traditional medicinal herb, has been highly valued for exhibiting antimicrobial, antioxidant and antispasmodic properties. Objective: For the first time, the synergetic anti-inflammatory effects of deuterium depleted water (DDW) and M. longifolia essential oils (ML) were investigated in experimental sepsis. Materials and methods: Fifty Wistar rats were divided into 5 groups (n = 10): negative control (laparotomy), CLP, treatment groups including the combination of DDWs (15 and 30 ppm) and ML (100 mg/kg b.w) and indomethacin. At 24 h after CLP induction, lipid peroxidation (LP), glutathione (GSH), glutathione in S-transferases (GST), ferric reducing ability of plasma (FRAP), myeloperoxidase (MPO), prostaglandin E2 (PGE2), and COX-2 expression were determined in the plasma and liver tissues. Results: Compared with the CLP group, the administration of DDWs and ML significantly (p < 0.05) prevented the increase of LP, COX-2 and PGE2 levels and liver enzymes. Additionally, the decreased levels of FRAP and GSH induced by sepsis were remarkably (p < 0.05) risen by the administration of DDWs and ML in comparison to the CLP group. However, no significant (p > 0.05) differences were observed regarding GST, ALP and bilirubin levels. Our results also proved the synergistic anti-inflammatory activities of the DDWs and ML. The anti-inflammatory effects of the DDWs and ML were confirmed by histopathological studies. Discussion and conclusions: The combination of DDWs and ML exerted synergistic anti-inflammatory activity against CLP-induced sepsis possibly through modulating oxidative stress/antioxidant parameters. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 CODEN: PHBIF Correspondence Address: Fatemi, F.; Materials and Nuclear Fuel Research School, Nuclear Science and Technology Research InstituteIran; email:
Uncontrolled Keywords: anti-inflammation gene expression histopathology Natural products oxidative stress/antioxidant parameters prostaglandin E2 alkaline phosphatase bilirubin cyclooxygenase 2 deuterium depleted water essential oil glutathione glutathione transferase indometacin myeloperoxidase unclassified drug water antiinflammatory agent antioxidant deuterium alkaline phosphatase blood level animal cell animal experiment animal model animal tissue antiinflammatory activity antioxidant activity Article bilirubin blood level controlled study drug dose comparison drug potentiation enzyme regulation ferric reducing antioxidant power assay lipid peroxidation liver injury liver level liver tissue male Mentha longifolia nonhuman oxidative stress protein blood level protein expression rat sepsis Wistar rat animal chemistry comparative study disease model drug effect isolation and purification liver disease Mentha metabolism Animals Anti-Inflammatory Agents Antioxidants Disease Models, Animal Drug Synergism Indomethacin Liver Diseases Oils, Volatile Rats Rats, Wistar
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 27 Jun 2020 05:26
Last Modified: 27 Jun 2020 05:26

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