Evaluating pre- and post-operation plasma miRNAs of papillary thyroid carcinoma (PTC) patients in comparison to benign nodules

Rezaei, M. and Khamaneh, A. M. and Zarghami, N. and Vosoughi, A. and Hashemzadeh, S. (2019) Evaluating pre- and post-operation plasma miRNAs of papillary thyroid carcinoma (PTC) patients in comparison to benign nodules. BMC Cancer, 19 (1).

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Abstract

Background: Thyroid cancer is the most common endocrinology cancer that its incidence has increased in recent decades. miRNAs are new biomarkers in recent studies in the diagnosis and follow-up of these patients. Methods: Blood and thyroid tissue samples were obtained from two groups of included patients (PTC and benign nodules), pre- and post-operation. miRNAs were extracted from these plasma samples and were measured quantitatively. After cDNA synthesis, qPCR was carried out. Then tissue samples were investigated, and their relation to miR expression was studied. These results were analyzed by paired- and independent samples t-test, and non-parametric tests. Results: miR-222 and miR-181a declined in PTC patients before and after surgery, significantly (P < 0.001 for both groups), with no significant difference in control group before and after surgery (P = 0.61 for miR-222 and P = 0.06 for miR-181a). The difference between the two groups, pre-and post-operation, was statistically significant (P = 0.01 for miR-222 and P < 0.001 for miR-181a). Comparing case and control groups, pre- and post-operatively, yielded no significant difference, in miR-155-5p levels (P = 0.61 and P = 0.53, respectively). Comparing PTC and control groups before surgery showed a significant difference (P = 0.01), while no significant difference was observed comparing them after surgery, in miR146-a (P = 0.27). Our results depicted a higher miR-155-5p and miR-146a expression before surgery than after it (P < 0.001 in both groups, for both miRs). We found a significant relationship between miR-222 and BRAFV600E mutation and significantly higher levels of miR-181a with increasing tumor size in PTC patients. Conclusion: miR-222 showed overexpression in all PTC cases, which is indicative of a relation between miRNA and PTC. Also, comparing miR-181 and miR-146a showed a significant difference between cancerous and benign cases. miR-155-5p as an inflammatory factor, showed no significant changes, comparing two groups. © 2019 The Author(s).

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 CODEN: BCMAC Correspondence Address: Vosoughi, A.; Neurosciences Research Center (NSRC), Faculty of Medicine, Tabriz University of Medical Sciences, Imam Reza Hospital, Golgasht Street, Iran; email: vosoughi954@gmail.com
Uncontrolled Keywords: Benign nodules MicroRNA Papillary thyroid carcinoma Plasma samples B Raf kinase complementary DNA microRNA 146a microRNA 155 5p microRNA 181a microRNA 222 unclassified drug transcriptome tumor marker adult Article blood level cancer patient cancer surgery clinical article controlled study female gene expression human human tissue male mutation postoperative period preoperative period thyroid papillary carcinoma tumor volume biopsy blood genetics middle aged pathology real time polymerase chain reaction thyroid nodule thyroid tumor Biomarkers, Tumor Humans MicroRNAs Real-Time Polymerase Chain Reaction Thyroid Cancer, Papillary Thyroid Neoplasms
Subjects: WK Endocrine System
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 27 Jun 2020 05:29
Last Modified: 27 Jun 2020 05:29
URI: http://eprints.mums.ac.ir/id/eprint/18611

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