Effects of carob fruit extract on spermatogenesis, antioxidant status, and apoptosis in adult Male mice

Sadat, S. S. and Mohammadi, S. and Sazegar, G. and Fazel, A. and Ebrahimzadeh, A. and Mobarhan, M. G. and Beheshti, F. and Attari, S. S. and Tavallaei, S. (2019) Effects of carob fruit extract on spermatogenesis, antioxidant status, and apoptosis in adult Male mice. Pharmaceutical Sciences, 25 (3). pp. 184-189.

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Abstract

Background: Certain herbs instigate spermatogenesis; in contrast, some herbs arrest the spermatogenesis cycle. Ceratonia siliqua (carob) is a plant with a strong antioxidant property. The aim of this study was to evaluate the effects of carob fruit extract on spermatogenesis, testicular apoptosis, and oxidative stress in adult male mice. Methods: Forty adult male mice were randomly divided into five groups: control, sham, and carob 1-3. The sham group was injected with normal saline and the carob 1-3 groups were injected with 200, 400, and 800 mg/kg of the carob fruit extract intraperitoneally for 14 days, respectively. At the end of the injection period, spermatogenesis, testicular apoptosis, and oxidative stress were examined. Results: The sperm parameters increased in the mice that received 200 mg/kg of carob compared to the sham group (p<0.05). There was a significant increase in the weight index of the epididymis in the carob 3 group in comparison to the sham group (p=0.01). The number of positive tunnel cells was not statistically significant between different groups (p>0.05). The level of malondialdehyde decreased in the carob 1 and carob 3 groups, but this reduction was not statistically significant (p>0.05). In addition, the statistical analysis showed a significant difference in the mean superoxide dismutase levels in the carob 2 and carob 3 groups in comparison to the sham group (p<0.001). The statistical analysis showed a significant increase in the mean level of the catalase enzyme in the carob 1 group in comparison to the sham (p=0.02), and carob 2 groups (p=0.008). Conclusion: The administration of 200 mg of the carob fruit extract for 14 days increased the testicular index as well as sperm parameters and decreased the level of oxidative stress in the testicular tissue of adult mice. ©2019 The Authors. This is an open access article and applies the Creative Commons Attribution (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers. on spermatogenesis, testicular apoptosis, and oxidative stress in adult male mice.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Mohammadi, S.; Neurogenic Inflammation Research Center, Mashhad University of Medical SciencesIran; email: mohammadish@mums.ac.ir
Uncontrolled Keywords: Apoptosis Ceratonia siliqua Mice Spermatozoa antioxidant Carob fruit extract catalase malonaldehyde plant extract superoxide dismutase unclassified drug adult animal experiment animal tissue Article Carob cell viability comparative study controlled study epididymis histopathology male mouse nonhuman oxidative stress semen analysis seminiferous tubule spermatocyte spermatogenesis spermatozoon count spermatozoon motility testis tissue traditional medicine
Subjects: WQ Obstetrics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 27 Jun 2020 05:34
Last Modified: 27 Jun 2020 05:34
URI: http://eprints.mums.ac.ir/id/eprint/18619

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