New Molecular Markers Distinguishing Fonsecaea Agents of Chromoblastomycosis

Schneider, G. X. and Gomes, R. R. and Bombassaro, A. and Zamarchi, K. and Voidaleski, M. F. and Costa, F. F. and Leão, A. C. R. and Lima, B. J. F. S. and Soley, B. S. and Colombo, I. R. and Cândido, G. Z. and Najafzadeh, M. J. and Sun, J. and de Azevedo, C. M. P. S. and Marques, S. G. and de Hoog, G. S. and Vicente, V. A. (2019) New Molecular Markers Distinguishing Fonsecaea Agents of Chromoblastomycosis. Mycopathologia, 184 (4). pp. 493-504.

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Abstract

The species belonging to the genus Fonsecaea are the main causative agents of chromoblastomycosis. The invasive potential of Fonsecaea differs significantly among its various sibling species. Moreover, the lack of clarity on the virulence and availability of precise markers to distinguish and detect Fonsecaea species is attributed to the different ways of dissemination and pathogenicity. Therefore, the present study aimed to propose new molecular tools to differentiate between sibling species causing chromoblastomycosis. We used an infection model of chromoblastomycosis in BALB/c to study species-specific molecular markers for the in vivo detection of Fonsecaea species in biological samples. Specific primers based on the CBF5 gene were developed for Fonsecaea pedrosoi, Fonsecaea monophora, Fonsecaea nubica, and Fonsecaea pugnacius. In addition, a padlock probe was designed for F. pugnacius based on ITS sequences. We also assessed the specificity of Fonsecaea species using in silico, in vitro, and in vivo assays. The results showed that markers and probes could effectively discriminate the species in both clinical and environmental samples, enabling bioprospecting of agents of chromoblastomycosis, thereby elucidating the infection route of the disease. © 2019, The Author(s).

Item Type: Article
Additional Information: Export Date: 16 February 2020 CODEN: MYCPA Correspondence Address: de Hoog, G.S.; Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Pathology, Federal University of ParanáBrazil; email: s.hoog@westerdijkinstitute.nl
Uncontrolled Keywords: CBF5 gene Chromoblastomycosis Fonsecaea Molecular detection Padlock probe RCA
Subjects: WB Practice of Medicine
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 27 Jun 2020 06:03
Last Modified: 27 Jun 2020 06:03
URI: http://eprints.mums.ac.ir/id/eprint/18632

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