A new tricyclic abietane diterpenoid from Salvia chloroleuca and evaluation of cytotoxic and apoptotic activities

Shakeri, A. and Delavari, S. and Ebrahimi, S. N. and Asili, J. and Emami, S. A. and Tayarani-Najaran, Z. (2019) A new tricyclic abietane diterpenoid from Salvia chloroleuca and evaluation of cytotoxic and apoptotic activities. Brazilian Journal of Pharmacognosy, 29 (1). pp. 30-35.

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Abstract

In this study, sahandone (1) and a new diterpenoid named sahandol II (2) were isolated from the roots of Salvia chloroleuca Rech. f. & Aellen, Lamiaceae. The absolute configurations of compounds 1 and 2 were assigned by comparison of experimental electronic circular dichroism spectra and comparing with published data. Cytotoxic and apoptotic evaluation of the isolated compounds and the methanol crude extract and its subfractions including petroleum ether, dichloromethane, ethyl acetate, n-butanol and aqueous fraction on two human prostate cancer cell lines and a breast cancer cell lines, showed that non-polar and semi-polar subfractions had the potent cytotoxic effect on PC3 cells with the IC 50 values of 24.19, 33.59, and 47.15 μg/ml, respectively. Sub-G1 peak in flow cytometry histogram of cells treated with petroleum ether, dichloromethane and ethyl acetate subfractions showed the induction of apoptosis. Change in the Bax/Bcl-2 ratio and cleavage of poly ADP-ribose polymerase were observed. © 2019 Sociedade Brasileira de Farmacognosia

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Tayarani-Najaran, Z.; Medical Toxicology Research Center, Mashhad University of Medical SciencesIran; email: tayaraninz@mums.ac.ir
Uncontrolled Keywords: Absolute configuration Cytotoxic Diterpenoids Lamiaceae NMR Salvia chloroleuca Western blot abietane derivative acetic acid ethyl ester dichloromethane doxorubicin nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase petroleum ether plant extract sahandol ii sahandone tricyclic abietane diterpenoid unclassified drug apoptosis Article carbon nuclear magnetic resonance cell viability circular dichroism column chromatography controlled study cytotoxicity flow cytometry flow rate human human cell IC50 polyacrylamide gel electrophoresis proapoptotic activity protein cleavage proton nuclear magnetic resonance reversed phase high performance liquid chromatography Salvia staining Western blotting
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 27 Jun 2020 05:37
Last Modified: 27 Jun 2020 05:37
URI: http://eprints.mums.ac.ir/id/eprint/18637

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