Preparation and characterization of a dry powder inhaler composed of PLGA large porous particles encapsulating gentamicin sulfate

Shiehzadeh, F. and Tafaghodi, M. and Laal-Dehghani, M. and Mashhoori, F. and Fazly Bazzaz, B. S. and Imenshahidi, M. (2019) Preparation and characterization of a dry powder inhaler composed of PLGA large porous particles encapsulating gentamicin sulfate. Advanced Pharmaceutical Bulletin, 9 (2). pp. 255-261.

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Abstract

Purpose: Direct delivery of aminoglycosides to the lungs was under extensive evaluations during the last decades. Because of large particle size, low density and porous structure, large porous particles (LPPs) are versatile carriers for this purpose. In this study, poly (lactic-co-glycolic acid) (PLGA) LPPs encapsulating gentamicin sulfate were prepared and in vitro characteristics of their freeze-dried powder as a dry powder inhaler (DPI) were evaluated. Methods: To prepare PLGA LPPs, a double emulsification-solvent evaporation method was optimized and gentamicin sulfate was post-loaded in the LPPs. In vitro characteristics including morphological features, thermal behavior, aerodynamic profile and cumulative drug release were evaluated by the scanning electron microscope (SEM), differential scanning calorimetry (DSC), next-generation cascade impactor (NGI) and Franz diffusion cell respectively. Results: The obtained results revealed that the preparation method was capable to produce spherical large homogenous highly porous particles. 94 of gentamicin sulfate released from LPPs up to 30 minutes. Mass median aerodynamic diameter (MMAD) and fine particle fraction (FPF) were 4.9 µm and 39 respectively. Conclusion: In this study, dry powder formulation composed of PLGA LPPs encapsulating gentamicin sulfate showed a promising in vitro behavior as a pulmonary delivery carrier. Improvements on the aerodynamic behavior and in vivo evaluations recommended for further developments. © 2019 The Author (s).

Item Type: Article
Additional Information: Cited By :1 Export Date: 16 February 2020 Correspondence Address: Tafaghodi, M.; Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical SciencesIran; email: tafaghodim@mums.ac.ir
Uncontrolled Keywords: Aminoglycosides Drug delivery systems Dry powder inhalers Gentamicin sulfate Large porous particles Lung PLGA gentamicin polyglactin Article controlled study differential scanning calorimetry drug delivery system drug release drug stability dry powder emulsion evaporation freeze drying in vitro study nanoencapsulation next generation sequencing particle size porosity process optimization scanning electron microscopy thermal analysis
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 27 Jun 2020 05:40
Last Modified: 27 Jun 2020 05:40
URI: http://eprints.mums.ac.ir/id/eprint/18646

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