Evaluating the protective role of ascorbic acid in malathion-induced testis tissue toxicity of male rats

Taherdehi, F. G. and Jalali, M. and Fazel, A. and Valokola, M. G. and Nikravesh, M. R. (2019) Evaluating the protective role of ascorbic acid in malathion-induced testis tissue toxicity of male rats. International Journal of Preventive Medicine, 10 (1). pp. 1-5.

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Background: Malathion is one of organophosphate pesticides that is widely used in agriculture and crops to control insects. Malathion affects body organs such as the reproductive system by inhibiting acetylcholinesterase activity and induction of oxidative stress. This study is aimed to investigate the effects of malathion on glutathione (GSH) and malondialdehyde (MDA) level in testis of male rats, as well as to study the protective role of Ascorbic Acid. Methods: In this study, 30 adult male Wistar rats weighing approximately 200–250 g were divided into 5 groups of 6 rats each. These groups include a control group (no intervention), sham (normal saline 0.9), experimental Group 1 (malathion 50 mg/kg), experimental Group 2 (Malathion 50 mg/kg + Ascorbic Acid 200 mg/kg), and experimental Group 3 (Ascorbic Acid 200 mg/kg). Malathion, solvents, and ascorbic acid were injected intraperitoneally. After 6 weeks, all groups were anesthetized, and the right testis was used to measure levels of MDA and GSH. MDA as a marker of lipid peroxidation and GSH content was used. Results: The results showed that malathion increased MDA level and decreased GSH level compared with the control group (P < 0.001). It was also found that administration of malathion in combination with ascorbic acid reduced MDA level and increased the GSH level. Conclusions: Malathion‑induced lipid peroxidation and oxidative stress in the testis of rats. In addition, it seems that ascorbic acid, due to its antioxidant capabilities, can improve malathion‑induced poisonous changes. © 2019 International Journal of Preventive Medicine.

Item Type: Article
Additional Information: Export Date: 16 February 2020 Correspondence Address: Nikravesh, M.R.; Department of Anatomy and Cell Biology, School of Medicine, Azadi Square, Mashhad University of Medical SciencesIran; email: nikraveshmr@mums.ac.ir
Uncontrolled Keywords: Ascorbic acid Glutathione Malathion Malondialdehyde Rats Testis malonaldehyde sodium chloride adult animal experiment animal tissue Article controlled study lipid peroxidation male nonhuman protein content rat testis tissue Wistar rat
Subjects: WB Practice of Medicine
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib1 lib1
Date Deposited: 27 Jun 2020 05:43
Last Modified: 27 Jun 2020 05:43
URI: http://eprints.mums.ac.ir/id/eprint/18668

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