Anti-inflammatory and protective investigations on the effects of Theranekron® "an alcoholic extract of the Tarantula cubensis" on wound healing of peritoneal in the rat: An in vivo comparative study

Adib-Hashemi, F. and Farahmand, F. and Hesari, S. F. and Rezakhaniha, B. and Fallah, E. and Fayyaz, A. F. and Dadpay, M. (2015) Anti-inflammatory and protective investigations on the effects of Theranekron® "an alcoholic extract of the Tarantula cubensis" on wound healing of peritoneal in the rat: An in vivo comparative study. Diagnostic Pathology, 10 (1).

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Abstract

Background: The present study sought to investigate the effects of Tarantula cubensis extract (TC; Theranekron®) on the histopathological scores of peritoneal wound healing after laparotomy in the rats. Methods: This study was designed to investigate the effects of Theranekron on the peritoneal wound healing after wound creation, on days9, 14, 19, 24 and 29 post-injury in rats. Twenty-four mature Wister-albino male rats were randomly divided into two groups. In the experimental group, TC was repeatedly injected subcutaneously (SC) over the lesion 9, 14,19 and 24 days after laparotomy, whereas the control group received only normal saline by subcutaneous injection and then the animal groups were euthanized9, 14, 19, 24, and 29 days after wounding respectively by intravenous injections of pentobarbital (50 mg/kg). Finally, assessment of the peritoneal wound healing between the groups was carried out by histopathologic data and statistical tests as Mann-Whitney U, Wilcoxon W and Z Results: Histopathological examination indicated significant improvement in angiogenesis, re-epithelialization and less inflammatory response in comparison to control and also, revealed matured, compact and parallel deposition of collagen fibrils on day 29. So, at long term, treatment reduced the inflammation and increased the quality and rate of wound re- epithelialization compared to controls(P < 0.05). Furthermore, excluding the control group, rats exhibited the most pronounced effect on wound closure, with the statistically significant improvement in wound healing being seen at post-operative day 29. Moreover, collagen content on days 24 and 29 in the test group was found to be higher than in the healthy group. To warp up, treated groups had a significant increase in peritoneal wound healing area compared to the control group on all days (P < 0.05). Conclusions: Our results suggested that Theranekron have delivered a novel therapeutic route for wound treatment in clinical practice. Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2958770714954315. © Adib-Hashemi et al.; licensee BioMed Central.

Item Type: Article
Additional Information: Cited By :2 Export Date: 16 February 2020 Correspondence Address: Dadpay, M.; Department of Pathology, Imam Reza Hospital, AJA University of Medical SciencesIran
Uncontrolled Keywords: Histopathology Rat Regeneration Theranekron Wound angiogenic factor antiinflammatory agent collagen protective agent spider venom angiogenesis animal disease model drug administration drug effects epithelization male metabolism pathology pathophysiology peritoneum retracted article subcutaneous drug administration surgery time factor vascularization Wistar rat wound healing Wounds and Injuries Angiogenesis Inducing Agents Animals Anti-Inflammatory Agents Disease Models, Animal Drug Administration Schedule Injections, Subcutaneous Neovascularization, Physiologic Protective Agents Rats, Wistar Re-Epithelialization Spider Venoms Time Factors
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 03 May 2020 03:44
Last Modified: 03 May 2020 03:44
URI: http://eprints.mums.ac.ir/id/eprint/18730

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