Protective effect of scutellaria litwinowii extract on serum/glucose- deprived cultured PC12 cells and determining the role of reactive oxygen species

Afsharzadeh, M. and Tayarani-Najaran, Z. and Zare, A. and Mousavi, S. H. (2012) Protective effect of scutellaria litwinowii extract on serum/glucose- deprived cultured PC12 cells and determining the role of reactive oxygen species. Journal of Toxicology, 2012.

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Abstract

Considering the wide, positive reporting of the role of reactive oxygen species in ischemic brain injury, searching for antioxidant drugs within herbal remedies is logical. In this study, the protective effects of Scutellaria litwinowii Bornm. Sint. on cell viability and reactive oxygen species production in cultured PC12 cells were investigated under serum/glucose-deprivation- induced cell death. After cells were seeded overnight, they were then deprived of serum/glucose for 24h. Cells were treated with different concentrations of S. litwinowii extract (7.75250μg/mL). Cell viability was quantitated by MTT assay, and intracellular reactive oxygen species production was measured by flow cytometry. Serum/glucose-deprivation induced significant cell death after 24h (P < 0.001). Treatment with S. litwinowii (7.75250μg/mL) reduced serum/glucose deprivation-induced cytotoxicity in PC12 cells after 24h. A significant increase in intracellular reactive oxygen species production was seen following serum/glucose deprivation (P < 0.001). S. litwinowii (62 and 125μg/mL, P < 0.01) treatment reversed the increased reactive oxygen species production following ischemic insult. This demonstrates that S. litwinowii extract protects PC12 cells against serum/glucose-deprivation-induced cell death by antioxidant mechanisms, which indicates the potential therapeutic application of S. litwinowii in managing cerebral ischemic and neurodegenerative disorders. © Copyright 2012 Maryam Afsharzadeh et al.

Item Type: Article
Additional Information: Cited By :7 Export Date: 16 February 2020 Correspondence Address: Mousavi, S.H.; Pharmacological Research Centre of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; email: sshadim@yahoo.com
Uncontrolled Keywords: antioxidant reactive oxygen metabolite Scutellaria litwinowii extract unclassified drug antioxidant activity article cell viability controlled study cytotoxicity flow cytometry priority journal quantitative analysis
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 11 May 2020 09:30
Last Modified: 11 May 2020 09:30
URI: http://eprints.mums.ac.ir/id/eprint/18732

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