Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues

Ajdarkosh, H. and Dadpay, M. and Yahaghi, E. and Pirzaman, E. R. and Fayyaz, A. F. and Darian, E. K. and Mokarizadeh, A. (2015) Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues. Diagnostic Pathology, 10 (1).

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Background: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, mainly due to its high rates of postoperative recurrence and metastasis. Please remove, it currently ranks as the third most common cause of cancer-related deaths. MiRNAs are a set of small, single-stranded, non-coding RNA molecules that negatively regulate gene expression at the post-transcriptional level. In this study, we demonstrated the down-regulation of miR-148a in HCC and non-cancerous tissues using qRT-PCR. Methods: Ninety six HCC samples and their noncancerous normal liver tissues were collected. Total mRNA including miRNA was extracted, and miR-148a expression was determined using qRT-PCR. Furthermore, the correlation between the miR-148a expression and clinicopathological parameters was investigated. Results: The result showed that reduction of miR-148a expression was associated with TNM stage, metastasis, and number of tumor nodes. Multivariate Cox proportional hazards model analysis showed that low expression of miR-148a was independently associated with recurrence of HCC in the current study. Moreover, our result showed that lower expression in tumor tissues in comparison with corresponding normal control tissues. Conclusion: Down-regulation of miR-148a is related to HCC carcinogenesis and deterioration of HCC. MicroRNA-148a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients. © 2015 Ajdarkosh et al.

Item Type: Article
Additional Information: Cited By :10 Export Date: 16 February 2020 Correspondence Address: Mokarizadeh, A.; Cellular and Molecular Research Center, Kurdistan University of Medical SciencesIran
Uncontrolled Keywords: microRNA MIRN148 microRNA, human tumor marker adult aged area under the curve biosynthesis down regulation erratum female gene expression regulation genetics human Kaplan Meier method liver cell carcinoma liver tumor male middle aged mortality pathology prognosis receiver operating characteristic retracted article retrospective study reverse transcription polymerase chain reaction tumor recurrence Area Under Curve Biomarkers, Tumor Carcinoma, Hepatocellular Down-Regulation Gene Expression Regulation, Neoplastic Humans Kaplan-Meier Estimate Liver Neoplasms MicroRNAs Neoplasm Recurrence, Local Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction ROC Curve
Subjects: WI Digestive System
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 04 May 2020 04:55
Last Modified: 04 May 2020 04:55

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