Structural properties of a viral orthologue of cellular CD200 protein: KSHV vOX2

Ali Amini, A. and Solovyova, A. S. and Sadeghian, H. and Blackbourn, D. J. and Rezaee, S. A. R. (2015) Structural properties of a viral orthologue of cellular CD200 protein: KSHV vOX2. Virology, 474. pp. 94-104.

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Kaposi's sarcoma-associated herpesvirus (KSHV) vOX2 is a cell surface glycoprotein expressed during viral lytic replication to suppress host inflammatory reactions. Here we have characterised vOX2 with biochemical, biophysical and bioinformatics tools and as a result propose a 3-dimensional model for vOX2 based on structural and functional homology with the PD-L1 protein. To validate this model, vOX2 was characterised by analytical ultracentrifugation (AUC) and circular dichroism spectroscopy (CD). The results identified the potential glycosylation sites and revealed that vOX2 is predominantly a beta-folded molecule with an RGD adhesion motif exposed on the C-terminal domain. The protein exists in monomer-dimer equilibrium similar to its IgV-type folded homologues, with 30-36 glycosylation and the molecular weight of the extracellular fragment of molecule is 32.0-33.6. kDa, much less than 50. kDa. Thus, the structural similarity to PD-L1 verifies its immunomodulatory potential and the RGD motif suggests an adhesive capacity. © 2014.

Item Type: Article
Additional Information: Cited By :4 Export Date: 16 February 2020 CODEN: VIRLA Correspondence Address: Rezaee, S.A.R.; Inflammation and Inflammatory Diseases Research Centre, Medical school, Mashhad University of Medical SciencesIran
Uncontrolled Keywords: CD200 Glycosylation Homology modelling KSHV RGD domain VOX2 CD200 antigen cell surface protein membrane protein programmed death 1 ligand 1 unclassified drug vOX2 protein antigens, CD200 CD274 protein, human hybrid protein leukocyte antigen virus protein animal cell Article biochemistry bioinformatics biophysics carboxy terminal sequence CHO cell line circular dichroism Human herpesvirus 8 immunomodulation molecular weight nonhuman orthology protein domain protein folding protein function protein glycosylation protein motif protein structure structural homology three dimensional imaging ultracentrifugation amino acid sequence animal biology chemical structure chemistry Cricetulus genetics human molecular genetics physiology protein conformation protein multimerization protein tertiary structure Animals Antigens, CD Antigens, CD274 Biophysical Phenomena CHO Cells Computational Biology Herpesvirus 8, Human Humans Models, Molecular Molecular Sequence Data Protein Structure, Tertiary Recombinant Fusion Proteins Structural Homology, Protein Viral Proteins
Subjects: WC Communicable Diseases
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 11 May 2020 19:35
Last Modified: 11 May 2020 19:35

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