Metastatic inhibitory and radical scavenging efficacies of saponins extracted from the brittle star (Ophiocoma erinaceus)

Amini, E. and Nabiuni, M. and Baharara, J. and Parivar, K. and Asili, J. (2015) Metastatic inhibitory and radical scavenging efficacies of saponins extracted from the brittle star (Ophiocoma erinaceus). Asian Pacific Journal of Cancer Prevention, 16 (11). pp. 4751-4758.

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Abstract

Echinodermata use saponins in chemical defense against pathogens and predators. The molecular mechanisms of antimetastatic effects of brittle star saponins are still unknown. The present study examined antioxidant capacity and invasive ability in HeLa carcinoma cells exposed to brittle star crude saponins. Discolorating methods with DPPH and ABTS and expression of SOD-2 with RT-PCR were used to estimate the antioxidant activity. The anti-invasive activity of extracted saponins was examined through adhesion of HeLa cells to extracellular matrix, wound healing and evaluation of the mRNA levels of MMP-2 and MMP-9 by real time-PCR. The results showed that extracted saponins had cytotoxicity against cervical cancer cells and ABTS and DPPH scavenging properties with IC50 values of 604.5, 1012 μg/ml, respectively. Further, we found that, in wound healing assay, brittle star saponins could prevent invasion of HeLa cells in a concentration dependent manner. Furthermore, cell adhesion assay demonstrated blockage of cell attachment to extracellular matrix with an IC50 concentration of 16.1μg/ml. The significant dose dependent down regulation of MMP-2 and MMP-9 in treated cells demonstrated that isolated saponins can decline tumor metastasis in vitro. The brittle star saponins remarkably prevented cervical cancer invasion and migration associated with down regulation of matrix metalloproteinase expression. Therefore, saponins could be suggested as an anti-invasive candidate against cervical cancer and an antioxidant as well.

Item Type: Article
Additional Information: Cited By :6 Export Date: 16 February 2020 Correspondence Address: Baharara, J.; Department of Animal Biology, School of Basic Science, Science and Research Branch, Islamic Azad UniversityIran
Uncontrolled Keywords: Aggression Antioxidant capacity Attachment Cervical cancer Echinoderm Saponins antioxidant messenger RNA plant extract saponin animal apoptosis cell adhesion cell motion cell proliferation chemistry drug effects female genetics human pathology real time polymerase chain reaction reverse transcription polymerase chain reaction tumor cell culture tumor invasion Uterine Cervical Neoplasms Western blotting Animals Antioxidants Blotting, Western Cell Movement Echinodermata Humans Neoplasm Invasiveness Plant Extracts Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Tumor Cells, Cultured
Subjects: WP Gynecology
QZ pathology-neoplasms-Genetics
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 11 May 2020 10:02
Last Modified: 11 May 2020 10:02
URI: http://eprints.mums.ac.ir/id/eprint/18747

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