Genetic characterization of a vancomycin-resistant Staphylococcus aureus isolate from the respiratory tract of a patient in a University Hospital in Northeastern Iran

Azimian, A. and Havaei, S. A. and Fazeli, H. and Naderi, M. and Ghazvini, K. and Samiee, S. M. and Soleimani, M. and Peerayeh, S. N. (2012) Genetic characterization of a vancomycin-resistant Staphylococcus aureus isolate from the respiratory tract of a patient in a University Hospital in Northeastern Iran. Journal of Clinical Microbiology, 50 (11). pp. 3581-3585.

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Emergence of vancomycin-intermediate Staphylococcus aureus (VISA) and vancomycin-resistant S. aureus (VRSA) strains has led to global concerns about treatments for staphylococcal infections. These strains are currently rare even though there is an upward trend in their reported incidence. Therefore, appropriate screening and epidemiological evaluation of VRSA strains can affect future global health care policies. Isolates of Staphylococcus aureus were obtained from various clinical samples and were then evaluated with agar screening, disk diffusion, and MIC methods to determine resistance to vancomycin and methicillin. After confirmation of the isolated VRSA strain, genetic analysis was performed by evaluating mecA and vanA gene presence, SCCmec, agr, and spa types, and toxin profiles. Multilocus sequence typing (MLST) and plasmid analysis were also performed. The VRSA strain was resistant to oxacillin (MIC of 128 μg/ml) and vancomycin (MIC of 512 μg/ml). Disk diffusion antimicrobial susceptibility tests showed resistance to oxacillin, vancomycin, levofloxacin, ciprofloxacin, trimethoprim-sulfamethoxazole, clindamycin, rifampin, and tetracycline. The isolate was susceptible to minocycline and gentamicin. PCRs were positive for the mecA and vanA genes. Other genetic characteristics include SCCmec type III, agr I, spa type t037, and sequence type (ST) 1283. The plasmid profile shows five plasmids with a size of ∼1.7 kb to >10 kb. The isolated VRSA strain was obtained from a critically ill hospitalized patient. Genetic analysis of this strain suggested that the strain was a methicillin-resistant S. aureus (MRSA) clone endemic in Asia that underwent some genetic changes, such as mutation in the gmk gene and acquisition of the vanA gene. Copyright © 2012, American Society for Microbiology. All Rights Reserved.

Item Type: Article
Additional Information: Cited By :61 Export Date: 16 February 2020 CODEN: JCMID Correspondence Address: Havaei, S.A.; Department of Pathobiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; email:
Uncontrolled Keywords: ciprofloxacin clindamycin cotrimoxazole gentamicin hydrocortisone ketoconazole levofloxacin meticillin metronidazole minocycline oxacillin rifampicin tetracycline vancomycin abdominal disease Acinetobacter baumannii adult agr gene antibiotic resistance antibiotic sensitivity article bacterial gene bacterial genetics bacterium isolate bronchial aspiration procedure case report controlled study genetic analysis human inflammation male mecA gene multilocus sequence typing nonhuman peritonitis plasmid polymerase chain reaction priority journal respiratory distress respiratory system SCCmec gene septicemia spa gene Staphylococcus infection vanA gene vancomycin resistant Staphylococcus aureus Anti-Bacterial Agents Bacterial Proteins Bacterial Toxins Carbon-Oxygen Ligases Hospitals, University Humans Iran Methicillin Resistance Microbial Sensitivity Tests Plasmids Staphylococcus aureus Vancomycin Resistance
Subjects: WC Communicable Diseases
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 12 May 2020 08:00
Last Modified: 12 May 2020 08:00

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