Pseudomonas aeruginosa flagellin as an adjuvant: Superiority of a conjugated form of flagellin versus a mixture with a human immunodeficiency virus type 1 vaccine candidate in the induction of immune responses

Delavari, S. and Sohrabi, M. and Ardestani, M. S. and Faezi, S. and Tebianian, M. and Taghizadeh, M. and Shajiei, A. and Hosseini, S. Y. and Moghaddampour, M. and Mahdavi, M. (2015) Pseudomonas aeruginosa flagellin as an adjuvant: Superiority of a conjugated form of flagellin versus a mixture with a human immunodeficiency virus type 1 vaccine candidate in the induction of immune responses. Journal of Medical Microbiology, 64 (11). pp. 1361-1368.

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Abstract

In the present study, the adjuvant activity of flagellin was compared, in the conjugated and mixed forms, against a peptide vaccine from human immunodeficiency virus type 1 (HIV-1) p24–Nef vaccine candidate. Mice were immunized with the HIV-1 p24–Nef peptide with flagellin in both conjugated and mixed forms. Lymphocyte proliferation, CTL activity, and IL-4 and IFN-γ cytokines were evaluated by bromodeoxyuridine, carboxyfluorescein succinimidyl ester and ELISA methods, respectively. At the same time, the frequency of IFN-γ-producing T-lymphocytes, as well as total and isotype-specific antibodies, were assessed by ELISPOT and indirect ELISA, respectively. Our experimental results showed that the immunized mice with the HIV-1 p24–Nef conjugated or mixed forms of flagellin led to increases in the proliferative responses and Th1 cytokine pattern. The conjugated form of vaccine led to increased CTL activity and a Th1 cytokine pattern of immune responses, as well as an IgM isotype of humoral responses in comparison with the mixed form. The flagellin-conjugated vaccine seems to be more potent in increasing vaccine immunogenicity. © 2015 The Authors.

Item Type: Article
Additional Information: Cited By :12 Export Date: 16 February 2020 CODEN: JMMIA Correspondence Address: Mahdavi, M.; Department of Immunology, Pasteur Institute of IranIran; email: mmahdavi@pasteur.ac.ir
Uncontrolled Keywords: adjuvant flagellin Gag protein gamma interferon Human immunodeficiency virus vaccine Nef protein peptide vaccine immunological adjuvant interleukin 4 virus antibody animal cell animal experiment animal model antibody response Article comparative study controlled study cytotoxicity assay drug conjugation enzyme linked immunosorbent assay enzyme linked immunospot assay female Human immunodeficiency virus 1 humoral immunity immune response interferon production lymphocyte proliferation mouse nonhuman priority journal Pseudomonas aeruginosa T lymphocyte animal Bagg albino mouse genetics HIV Infections human immunology molecular genetics virology Adjuvants, Immunologic AIDS Vaccines Animals Antibodies, Viral HIV-1 Humans Interferon-gamma Interleukin-4 Mice Mice, Inbred BALB C Molecular Sequence Data
Subjects: WC Communicable Diseases
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 12 May 2020 05:29
Last Modified: 12 May 2020 05:29
URI: http://eprints.mums.ac.ir/id/eprint/18815

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