Improving multi-epitope long peptide vaccine potency by using a strategy that enhances CD4+ T Help in BALB/c mice

Ghaffari-Nazari, H. and Tavakkol-Afshari, J. and Jaafari, M. R. and Tahaghoghi-Hajghorbani, S. and Masoumi, E. and Jalali, S. A. (2015) Improving multi-epitope long peptide vaccine potency by using a strategy that enhances CD4+ T Help in BALB/c mice. PLoS ONE, 10 (11).

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Abstract

Peptide-based vaccines are attractive approaches for cancer immunotherapy; but the success of these vaccines in clinical trials have been limited. Our goal is to improve immune responses and anti-tumor effects against a synthetic, multi-epitope, long peptide from rat Her2/neu (rHer2/neu) using the help of CD4+ T cells and appropriate adjuvant in a mouse tumor model. Female BALB/c mice were vaccinated with P5+435 multi-epitope long peptide that presents epitopes for cytotoxic T lymphocytes (CTL) in combination with a universal Pan DR epitope (PADRE) or CpG-oligodeoxynucleotides (CpG-ODNs) as a Toll-like receptor agonist adjuvant. The results show that vaccination with the multi-epitope long peptide in combination with the PADRE peptide and CpG-ODN induced expansion of subpopulations of CD4+ and CD8+ cells producing IFN-Î, the average tumor size in the vaccinated mice was less than that of the other groups, and tumor growth was inhibited in 40 of the mice in the vaccinated group. The mean survival time was 82.6 ± 1.25 days in mice vaccinated with P5+435 + CpG+ PADRE. Our results demonstrate that inclusion of PADRE and CpG with the peptide vaccine enhanced significant tumor specific-immune responses in vaccinated mice. © 2015 Ghaffari-Nazari et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Item Type: Article
Additional Information: Cited By :22 Export Date: 16 February 2020 CODEN: POLNC
Uncontrolled Keywords: alanyllysylphenylalanylvalylalanylalanyltryptophylthreonylleucyllysylalanylalanylalanine CpG oligodeoxynucleotide gamma interferon peptide vaccine synthetic peptide unclassified drug cancer vaccine epitope subunit vaccine adjuvant therapy animal cell animal experiment animal model antineoplastic activity Article cancer inhibition CD4+ T lymphocyte CD8+ T lymphocyte cell expansion controlled study cytotoxic T lymphocyte drug efficacy drug potency female immune response immunization lymphocyte subpopulation monotherapy mouse nonhuman survival time tumor volume animal Bagg albino mouse immunology Mammary Neoplasms, Experimental Animals Cancer Vaccines CD4-Positive T-Lymphocytes Epitopes, T-Lymphocyte Mice Mice, Inbred BALB C T-Lymphocytes, Cytotoxic Vaccines, Subunit
Subjects: QV pharmacology
Divisions: Mashhad University of Medical Sciences
Depositing User: mr lib5 lib5
Date Deposited: 09 May 2020 06:57
Last Modified: 09 May 2020 06:57
URI: http://eprints.mums.ac.ir/id/eprint/18848

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